<p><b>a.</b> Immunoblotting of caspase 3 and PARP cleavages, <b>b.</b> H3 acetylation at day 25 and 30 in untreated (-) or treated (+) animals with VPA or/and PIO as indicated. *<i>As negative control of day 30 was used the BM flush of the last survived mouse of the group</i>, <i>with fully developed MM disease that was euthanized at day 25</i>. <b>c.</b> Effect of the combination of PIO with other HDACi on the H3 acetylation. MOLP8 cell line, treated with each of the three different HDACi (MGCD 0.2μM, SAHA 0.25μM and VPA 1mM) alone or combined with PIO 30μM. <b>d.</b> Study of the level of H3 acetylated of MOLP8 cells after treatment with the combination HDAC inhibitors/PIO and with the combination HDAC inhibitors/ PIO /PPARγ antagonist. Partial inhibition of H3 acetylation can be observed, by the pharmacological PPARγ antagonist, BADGE (0.5μM), or by genetic approaches using siGFP (as positive control) and siPPARγ after 48 hours of treatment.</p

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Last time updated on 12/02/2018

This paper was published in FigShare.

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