The natural product prodigiosin binds G-quadruplex DNA

Abstract

<p>Prodigiosin, a metabolite isolated from <i>Serratia</i> bacteria, is a potent anti-cancer agent. The goal of this research was to determine if prodigiosin could bind to G-quadruplex DNA. We found by using UV–vis spectroscopy that the G-quadruplex K<sup>+</sup>√d[TG<sub>4</sub>T]<sub>4</sub>, but not the single-stranded Li<sup>+</sup>√d[TG<sub>4</sub>T], increased the water solubility of prodigiosin. In addition, we found that prodigiosin's fluorescence was strongly enhanced in the presence of K<sup>+</sup>√d[TG<sub>4</sub>T]<sub>4</sub>, further evidence for quadruplex-ligand binding. Using <sup>1</sup>H and <sup>31</sup>P NMR experiments, we confirmed that prodigiosin binds at the 3′-end of K<sup>+</sup>√d[TG<sub>4</sub>T]<sub>4</sub>. Saturation transfer difference NMR experiments, done by irradiating the G5 H8 signal in K<sup>+</sup>√d[TG<sub>4</sub>T]<sub>4</sub>, indicated specific interactions between the G-quadruplex and prodigiosin's A and B rings. This study raises the possibility that G-quadruplex DNA may well be a target for the anti-cancer agent prodigiosin.</p

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Last time updated on 12/02/2018

This paper was published in FigShare.

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