Cytokine profiles in the distal colon of colitic mice correlate with increased CD103⁺CD11b⁺ P2 DCs in the same colon segment.

Abstract

<p>LP cells of the other half of the same distal colon segment of the same mice used for cytokine analyses in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.g003" target="_blank">Fig 3</a> were analyzed by flow cytometry. Cells were gated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130750#pone.0130750.g002" target="_blank">Fig 2A</a>. (A-C) The frequency of DCs belonging to subsets P1 (A), P2 (B) or P3 (C) among viable MHCII⁺CD11c^hi cells is shown. (D-E) The absolute number of DCs belonging to subsets P1 (D), P2 (E) or P3 (F) among 10⁶ viable LP cells is depicted. Each symbol represents an individual mouse and the solid line indicates the mean of each group. Statistical significance between groups was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test.</p