<p>As shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131477#pone.0131477.g002" target="_blank">Fig 2</a>, T cells were electroporated with NKT-TCR mRNA. (A) Six hours after NKT-TCR mRNA electroporation, Vα24<sup>+</sup>Vβ11<sup>+</sup> cells (left panel) and Vα24<sup>-</sup>Vβ11<sup>-</sup> cells (right panel) were sorted. (B) Each population was exposed to plate-bound CD1d-dimer loaded with α-GalCer for the indicated periods of times. As positive control, PBMCs were activated with PMA (50 ng/ml) and Ionomycin (1 μg/ml) for 5 min. Cells were then lysed and pERK1/2 and ERK1/2 were detected by Western blotting (upper panel). The bar diagram shows a densitometric analysis of the phosphorylated ERK signal from the upper band normalized by the corresponding total ERK signal (lower panel). (C) Sorted 1x10<sup>5</sup> Vα24<sup>+</sup>Vβ11<sup>+</sup> cells (top), Vα24<sup>-</sup>Vβ11<sup>-</sup> cells (middle) or NKT line (bottom) were cocultured with 1x10<sup>4</sup> CD1d-HEK293 loaded with or without α-GalCer for 24 h. IFN-γ and IL-4 production in the culture supernatants was analyzed by ELISA. Data are mean ±SEM from triplicates and representative of 5 healthy donors with similar results. (***p<0.005 for CD1d-HEK293/Gal vs. CD1d-HEK293/non and T only)</p

Similar works

Full text



Last time updated on 12/02/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.