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Population Genetics and Antimicrobial Susceptibility of Canine <i>Campylobacter</i> Isolates Collected before and after a Raw Feeding Experiment

By Satu Olkkola (769764), Sara Kovanen (769765), Johanna Roine (769766), Marja-Liisa Hänninen (183677), Anna Hielm-Björkman (147885) and Rauni Kivistö (769767)


<div><p>In recent years, increasing numbers of consumers have become interested in feeding raw food for their pet dogs as opposed to commercial dry food, in the belief of health advantages. However, raw meat and internal organs, possibly contaminated by pathogens such as <i>Campylobacter</i> spp., may pose a risk of transmission of zoonoses to the pet owners. <i>Campylobacter jejuni</i> is the leading cause of bacterial gastroenteritis in humans but <i>C</i>. <i>upsaliensis</i> has also been associated with human disease. In this study we investigated the effect of different feeding strategies on the prevalence of <i>Campylobacter</i> spp. in Finnish dogs. We further characterized the isolates using multilocus sequence typing (MLST), whole-genome (wg) MLST and antimicrobial susceptibility testing. Dogs were sampled before and after a feeding period consisting of commercial raw feed or dry pellet feed. Altogether 56% (20/36) of the dogs yielded at least one <i>Campylobacter</i>-positive fecal sample. <i>C</i>. <i>upsaliensis</i> was the major species detected from 39% of the dogs before and 30% after the feeding period. Two <i>C</i>. <i>jejuni</i> isolates were recovered, both from raw-fed dogs after the dietary regimen. The isolates represented the same genotype (ST-1326), suggesting a common infection source. However, no statistically significant correlation was found between the feeding strategies and <i>Campylobacter</i> spp. carriage. The global genealogy of MLST types of dog and human <i>C</i>. <i>upsaliensis</i> isolates revealed weakly clonal population structure as most STs were widely dispersed. Major antimicrobial resistance among <i>C</i>. <i>upsaliensis</i> isolates was against streptomycin (STR MIC > 4mg/l). Apart from that, all isolates were highly susceptible against the antimicrobials tested. Mutations were found in the genes <i>rpsL</i> or <i>rpsL</i> and <i>rsmG</i> in streptomycin resistant isolates. In conclusion, increasing trend to feed dogs with raw meat warrants more studies to evaluate the risk associated with raw feeding of pets in transmission of zoonoses to humans.</p></div

Topics: Biological Sciences, mic, upsaliensi, Campylobacter spp, clonal population structure, feed, Raw Feeding Experiment, multilocus sequence typing, str, st, mlst, Canine Campylobacter Isolates Collected, antimicrobial susceptibility testing, Major antimicrobial resistance
Year: 2015
DOI identifier: 10.1371/journal.pone.0132660
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Provided by: FigShare
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