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Endogenous and exogenous expression of genes specific to undifferentiated ESCs in biPSCs.

By Takamasa Kawaguchi (785125), Tomoyuki Tsukiyama (542302), Koji Kimura (435476), Shuichi Matsuyama (671501), Naojiro Minami (435479), Masayasu Yamada (785126) and Hiroshi Imai (435478)

Abstract

<p>mRNA expression was evaluated by reverse-transcription polymerase chain reaction (RT-PCR). pbiPSCs (P5), primed-type iPSCs at passage 5; pbiPSCs (P50), primed-type iPSCs at passage 50; pnbiPSCs, naïve-type iPSCs at passage 3 converted from primed-type iPSCs at passage 48; nbiPSCs, naïve-type iPSCs cultured under naïve medium from primary culture; bADCs, bovine amnion-derived cells; SNL feeder, SNL feeder cells; vector, plasmid DNA of PB vectors; bACT, bovine β-ACTIN specific for cattle; Uni-ACT, universal β-ACTIN that reacts with both cattle and mice.</p

Topics: Biological Sciences, stella, FGF 5 expression, ESRR, ksr, oct, xist, GSK 3 inhibitor, ïve biPSCs, rex, nanog, type, mouse PSCs, socs, ïve mouse PSCs, knockout serum replacement, icm
Year: 2015
DOI identifier: 10.1371/journal.pone.0135403.g004
OAI identifier: oai:figshare.com:article/1514795
Provided by: FigShare
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