Endogenous and exogenous expression of genes specific to undifferentiated ESCs in biPSCs.

Abstract

<p>mRNA expression was evaluated by reverse-transcription polymerase chain reaction (RT-PCR). pbiPSCs (P5), primed-type iPSCs at passage 5; pbiPSCs (P50), primed-type iPSCs at passage 50; pnbiPSCs, naïve-type iPSCs at passage 3 converted from primed-type iPSCs at passage 48; nbiPSCs, naïve-type iPSCs cultured under naïve medium from primary culture; bADCs, bovine amnion-derived cells; SNL feeder, SNL feeder cells; vector, plasmid DNA of PB vectors; bACT, bovine β-ACTIN specific for cattle; Uni-ACT, universal β-ACTIN that reacts with both cattle and mice.</p