SDS-PAGE of purified membrane protein by Ni-affinity chromatography in different imidazole concentration.


<p>The imidazole concentration in the elution buffer was optimized at 3 levels: 75 (A), 125 (B), and 250 Mm (C). When it reached 250 mM, the eluate in 7-10 tubes contained abundant recombinant β<sub>2</sub>-AR protein.</p

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oaioai:figshare.com:article/1560302Last time updated on 2/12/2018

This paper was published in FigShare.

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