Article thumbnail
Location of Repository

Analysis of H-ferritin and holo-transferrin uptake by hematopoietic cell lines.

By Soichiro Sakamoto (803735), Hiroshi Kawabata (570229), Taro Masuda (684825), Tatsuki Uchiyama (803736), Chisaki Mizumoto (803737), Katsuyuki Ohmori (803738), H. Phillip Koeffler (75276), Norimitsu Kadowaki (510500) and Akifumi Takaori-Kondo (510501)

Abstract

<p>(A) Uptake of AF488-labeled human holo-transferrin (Tf), H-ferritin (HFt), and L-ferritin (LFt). Cells were incubated with AF488-labeled ferritins (11 nM) or Tf (62 nM) for 60 min and analyzed by flow cytometry. The MFI ratio was defined as the MFI of cells treated with fluorescence-labeled ligand divided by the MFI of untreated cells. Filled columns denote the results of competition experiments using a 20-fold excess of unlabeled cognate ligand. Graphs for NB4, Reh, and THP–1 cell lines are shown on a larger scale in the in the enclosed areas. Data represent the mean ± standard error of three independent experiments. (B) Relationship between HFt and holo-Tf uptake. Cell lines shown in (A) are shown as blue dots by their MFI ratios for HFt and holo-Tf uptake in a scatter plot (Spearman’s coefficient, ρ = 0.95; P < 0.0001). A red dot in this plot represents the position of human bone marrow erythroblasts shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139915#pone.0139915.g003" target="_blank">Fig 3B</a>.</p

Topics: Uncategorised, hematopoietic cell lines, Human Erythroid Cells, uptake, Chinese hamster ovary cells, blood cell types, erythroid cells, rgd, hematopoietic cells, cell surface TFR 1 expression, Tfr 1 expression, threshold level, TFR 1, bone marrow erythroblasts, Transferrin Receptor 1
Year: 2015
DOI identifier: 10.1371/journal.pone.0139915.g002
OAI identifier: oai:figshare.com:article/1565892
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • http://www.plosone.org/article... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.