Intestinal mucositis is dependent on MyD88 and NF-κB expression.

Abstract

<p>The mice (n = 6–9) were injected for 4 days with saline (3 mL/kg, open bars) or irinotecan (75 mg/kg, i.p., black bars) and were killed on the seventh day after the first dose. Ileal samples were collected and processed for MyD88 and NF-κB expression. Irinotecan injection increased MyD88 (<b>A</b>) and NF-κB (<b>B, C and D</b>) expression as detected by qPCR (<b>A and C</b>), immunohistochemistry (<b>B</b>) or western blot (<b>D</b>) versus the saline-treated WT mice. The basal expression of MyD88 was found in the irinotecan-injected MyD88-/-, TLR2-/- or TLR9-/- mice when compared to their respective saline-injected knockout controls (<b>A</b>). Deletion of the MyD88 gene also prevented the expression of NF-κB (<b>B, C and D</b>). The values are expressed as the means ± SEM.</p