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Intestinal mucositis is dependent on MyD88 and NF-κB expression.

By Deysi V. T. Wong (803817), Roberto C. P. Lima-Júnior (803818), Cibele B. M. Carvalho (803819), Vanessa F. Borges (803820), Carlos W. S. Wanderley (803821), Amanda X. C. Bem (803822), Caio A. V. G. Leite (803823), Maraiza A. Teixeira (803824), Gabriela L. P. Batista (803825), Rangel L. Silva (788968), Thiago M. Cunha (276874), Gerly A. C. Brito (468496), Paulo R. C. Almeida (803826), Fernando Q. Cunha (276880) and Ronaldo A. Ribeiro (670473)

Abstract

<p>The mice (n = 6–9) were injected for 4 days with saline (3 mL/kg, open bars) or irinotecan (75 mg/kg, i.p., black bars) and were killed on the seventh day after the first dose. Ileal samples were collected and processed for MyD88 and NF-κB expression. Irinotecan injection increased MyD88 (<b>A</b>) and NF-κB (<b>B, C and D</b>) expression as detected by qPCR (<b>A and C</b>), immunohistochemistry (<b>B</b>) or western blot (<b>D</b>) versus the saline-treated WT mice. The basal expression of MyD88 was found in the irinotecan-injected MyD88-/-, TLR2-/- or TLR9-/- mice when compared to their respective saline-injected knockout controls (<b>A</b>). Deletion of the MyD88 gene also prevented the expression of NF-κB (<b>B, C and D</b>). The values are expressed as the means ± SEM.</p

Topics: Uncategorised, wt, cox, adaptor protein MyD 88, TLR 9, 57BL, mpo, Mucositis causes cell damage, nf, Key Signaling Molecule, traf, MyD 88, Myeloid Differentiation adaptor protein, il
Year: 2015
DOI identifier: 10.1371/journal.pone.0139985.g004
OAI identifier: oai:figshare.com:article/1566141
Provided by: FigShare
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