<p>(A) 293T cells were transfected with p3XFLAG-CMV7.1-hRpn2 (916–953) WT or F948Stop plasmids and the cell lysates immunoprecipitated with anti-hRpt3 antibodies and immunoprobed with antibodies against hRpn13, hRpt3, and hRpn2, as indicated. (B) Cell lysates from 293T cells transfected with HA-ubiquitin alone or together with either p3XFLAG-CMV7.1-hRpn2 (916–953) WT or F948Stop plasmids were immunoblotted with anti-ubiquitin and anti-β-actin antibodies, as indicated. (C) A model illustrating hRpn2 (916–953) peptide interacting with hRpn13 and displacing it from the proteasome.</p
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