Effect of AmpliconDuo filtering on chimeras for prokaryotic sample Pro2.


<p>Chimeras defined by being recognized by UCHIME in <i>de novo</i> mode with score ≥ 1. Top: Frequency of chimeras in branches A, B of split sample as function of their read numbers, before (dashed lines) and after (solid lines) application of AmpliconDuo filter. Bottom: Fraction of chimeras passing the AmpliconDuo filter (<i>f</i><sub><i>filtered</i></sub>/<i>f</i><sub><i>unfiltered</i></sub>) for read numbers 1 to 20 in both branches A, B, and corresponding prediction <i>P</i>(<i>r</i><sub><i>iA</i></sub>, <i>r</i><sub><i>iB</i></sub> ≥ 1) using the Poisson model in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141590#pone.0141590.e026" target="_blank">Eq (8)</a> with <i>λ</i><sub><i>i</i></sub> = 1, 2, …, 20.</p

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oai:figshare.com:article/1591254Last time updated on 2/12/2018

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