Overlapping IgE repertoires in different organs after primary and secondary <i>N</i>. <i>brasiliensis</i> infection.
<p>(A) Heat maps for the first 50 most frequent CDR3 sequences in the IgE repertoires from bone marrow (BM), lung (LU), spleen (SP), and lymph nodes (LN) of one exemplary <i>N</i>. <i>brasiliensis</i>-infected mouse after primary (1st) and secondary infection (2nd). Each row indicates one unique CDR3 sequence ordered by decreasing frequency in the IgE pools of BM, LU, SP, and LN (left to right). The brightest green indicates an abundance of ≥1% of a particular CDR3 sequence. (B) Morisita-Horn indices show the relatedness between 1,000 randomly chosen CDR3 sequences of IgE in the indicated organs after primary and secondary infection. (C) Number of different CDR3 in 1,000 randomly chosen IgE sequences from indicated organs after primary and secondary infection. (D) Frequencies of SHMs in V<sub>H</sub> regions of IgE in indicated organs after primary and secondary infection. Data in (B) and (C) show the mean + SEM from two mice per experiment.</p