Abstract

<p>(A) Histological analysis by H&E staining of large intestine of control, ATM<sup>-/-</sup>, ATMIN<sup>ΔL</sup>, NBS1<sup>ΔL</sup>, ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> mice at 12 weeks of age. (B) Histological scores of the large intestine of control, ATM<sup>-/-</sup>, ATMIN<sup>ΔL</sup>, NBS1<sup>ΔL</sup>, ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> and 3 individual moribund ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> mice. (C) Histological analysis by anti-CD3 staining of the large intestine of control and a moribund ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> mouse. (D) Representative flow cytometry data of CD4 and CD8 expression, as well as (E) TCRβ and TCRγδ expression on isolated IELs from the small intestine of control, ATMIN<sup>ΔL</sup>, NBS1<sup>ΔL</sup> and ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> mice, along with the quantification of D-E. N = 5–8 mice per genotype. (F) Representative flow cytometry data of IL17A and IFNγ production by YFP<sup>-</sup> and YFP<sup>+</sup> IELs (gated on the CD4<sup>+</sup> population) isolated from the small intestine of control and ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> mice after PMA and ionomycin stimulation. (G) Large intestinal sections from control, ATM<sup>-/-</sup>, ATMIN<sup>ΔL</sup>, NBS1<sup>ΔL</sup> and ATMIN<sup>ΔL</sup>NBS1<sup>ΔL</sup> mice were stained for γH2AX and DAPI. Error bars represent SEM (**<i>P</i><0.01, ***<i>P</i><0.001).</p

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oai:figshare.com:article/1596243Last time updated on 2/12/2018

This paper was published in FigShare.

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