Indirubin increased the levels of CD4⁺CD25⁺T cells in spleens of ITP mice.

Abstract

<p>The total cell number (<b>A</b>) and the percentages of CD4⁺T cells (<b>B</b>) in the spleens were unchanged in ITP and indirubin-treated ITP mice. <b>C</b>, Representative FACS analysis of CD4⁺CD25⁺ cells in spleens was shown. <b>D</b>, Indirubin significantly increased the percentages of CD4⁺CD25⁺Treg cells in the spleens of ITP mice. <b>E</b>, Representative FACS analysis of Foxp3 expression in gated CD4⁺CD25⁺ cells in spleens was shown <b>F</b>, Indirubin significantly increased the percentage of CD4⁺CD25⁺Foxp3⁺Treg cells in ITP mice. Data are representative of 3 independent experiments. *** P<0.001 compared with control or ITP mice.</p