10.1371/journal.pone.0144810.g005

Antagomir efficiency varies across immune cell populations.

Abstract

<p>Isolated lung cells were incubated with fluorescently (DY547)-labeled Ant at indicated concentrations for 1 hour. Mean fluorescent intensity and percentage of DY547<sup>+</sup> cells for granulocytes (SSC<sup>hi</sup>), B220<sup>+</sup> and CD4<sup>+</sup> cells (A). CD4<sup>+</sup> T cells were CFSE-labeled and cultured with 5 μM Ant for 3 days. Representative histogram plots and proliferation analysis for unlabeled and unstimulated, CFSE-labeled controls (Controls), PBS-treated (PBS) and Ant-treated (either Scramble or Ant-155) CD4<sup>+</sup> T cells (B). Representative graphs from 3 independent experiments. Error bars SEM (n = 3 replicates/group). Fluorescently-labeled Ant uptake by lung immune cells <i>in vivo</i> following intranasal Ant administration (C). Mean fluorescent intensity and percentage of DY547<sup>+</sup> cells for CD11c<sup>+</sup>, F4/80<sup>+</sup>, Ly6G<sup>+</sup>, B220<sup>+</sup> and CD4<sup>+</sup> cell populations.</p

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oai:figshare.com:article/1628871Last time updated on 2/12/2018

This paper was published in FigShare.

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