10.1371/journal.pone.0146245.g005

IL-2 complexes relocate B cells from the bone marrow to secondary lymphoid organs.

Abstract

<p>The mechanisms of B cell expansion in secondary lymphoid organs were investigated. Naive C57BL/6 mice received IL-2 complexes (5μg IL-2 / 25μg α-IL-2) for three days (d0, d1, d2) and distinct cell populations were analyzed in indicated tissues 2 days (d4) after the last dose. <b>(A)</b> CD3<sup>+</sup> CD4<sup>+</sup> Foxp3<sup>−</sup> T cells, CD3<sup>+</sup> CD8<sup>+</sup> T cells, CD3<sup>−</sup> Nk1.1<sup>+</sup> NK cells and CD3<sup>+</sup> NK1.1<sup>+</sup> cells but not CD19<sup>+</sup> B cells proliferated upon IL-2 complex treatment in the spleen as assessed by their expression of Ki67. Histograms are shown from representative mice (n = 4). <b>(B)</b> IL-2 complexes (n = 4) reduced the proportion and absolute number of CD19<sup>+</sup> B cells within CD45<sup>+</sup> leukocytes in the BM as compared with untreated mice (n = 4). Two color dot plots illustrate representative mice (left). Data are pooled from 2 independent experiments (right) [p = 0.014].</p

Similar works

Full text

thumbnail-image
oai:figshare.com:article/1633304Last time updated on 2/12/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.