IL-2 complexes relocate B cells from the bone marrow to secondary lymphoid organs.


<p>The mechanisms of B cell expansion in secondary lymphoid organs were investigated. Naive C57BL/6 mice received IL-2 complexes (5μg IL-2 / 25μg α-IL-2) for three days (d0, d1, d2) and distinct cell populations were analyzed in indicated tissues 2 days (d4) after the last dose. <b>(A)</b> CD3<sup>+</sup> CD4<sup>+</sup> Foxp3<sup>−</sup> T cells, CD3<sup>+</sup> CD8<sup>+</sup> T cells, CD3<sup>−</sup> Nk1.1<sup>+</sup> NK cells and CD3<sup>+</sup> NK1.1<sup>+</sup> cells but not CD19<sup>+</sup> B cells proliferated upon IL-2 complex treatment in the spleen as assessed by their expression of Ki67. Histograms are shown from representative mice (n = 4). <b>(B)</b> IL-2 complexes (n = 4) reduced the proportion and absolute number of CD19<sup>+</sup> B cells within CD45<sup>+</sup> leukocytes in the BM as compared with untreated mice (n = 4). Two color dot plots illustrate representative mice (left). Data are pooled from 2 independent experiments (right) [p = 0.014].</p

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oai:figshare.com:article/1633304Last time updated on 2/12/2018

This paper was published in FigShare.

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