Abstract

<p>Cryopreserved rhesus macaque PBMCs were thawed, phenotyped and assessed for function. (A) Graphs show frequency (%) of rhTIGIT<sup>+</sup>CD8<sup>+</sup> T cells from PBMCs (circle), LNs (square), and spleen (triangle) in SIV-uninfected (filled) and SIV-infected (open) animals (SIV-uninfected PBMCs, <i>n</i> = 8; SIV-infected PBMCs, <i>n</i> = 19; SIV-uninfected LNs, <i>n</i> = 8; SIV-infected LNs, <i>n</i> = 22; SIV-uninfected spleen, <i>n</i> = 6; SIV infected spleen, <i>n</i> = 9). P values were calculated with Mann-Whitney U tests. (B) Graphs show correlation of frequency (%) of rhTIGIT<sup>+</sup> CD8<sup>+</sup> T cells in PBMCs (circle) and lymph nodes (square) from SIV-infected animal against plasma SIV viral load log<sub>10</sub> vRNA copy Eq/ml (PBMCs, <i>n</i> = 12; LNs, <i>n</i> = 20). vRNA copy Eq, viral RNA copy equivalents. Spearman’s rho tests were performed for correlations. (C) Representative flow cytometry plots of tetramer stains for Mamu-A*01 restricted SIV-Gag CM9 and SIV-Tat SL8 specific CD8<sup>+</sup> T cells from PBMC, LNs, and spleen, in a representative Mamu-A*01 animal with full cART suppression. Compiled data of rhTIGIT expression frequency (%) on tetramer specific CD8<sup>+</sup> T cells (<i>n</i> = 4) from PBMCs (circle), LNs (square), and spleen (triangle) from Mamu-A*01<sup>+</sup> macaques with full cART suppression. (D) Representative flow cytometry plots of PBMCs (<i>n</i> = 12) or lymph nodes (<i>n</i> = 18) stimulated without or with PMA + Ionomycin. Graphs show frequency (%) of IFN-γ from CD8<sup>+</sup> T cells expressing TIGIT or not expressing TIGIT. P values were calculated by Wilcoxon matched-pairs signed ranked test. (E) Representative flow cytometry plots of PBMCs from a Mamu-A*01+ macaque stimulated without or with SIV-Gag<sub>181-189</sub> CM9 peptide. Graph shows frequency (%) of IFN-γ from SIV-Gag<sub>181-189</sub> CM9-specific CD8<sup>+</sup> T cells expressing TIGIT or not expressing TIGIT from Mamu-A*01<sup>+</sup> macaques (<i>n</i> = 6). P values were calculated by Wilcoxon matched-pairs signed ranked test. (F) Representative flow cytometry plots of CD8<sup>+</sup> T cells from two separate SIV-infected macaques showing CD8<sup>+</sup> T cell CFSE dilution in response to AT2-inactivated SIV with either no antibody, anti-TIGIT, anti-PD-L1 or dual blockade (anti-TIGIT + anti-PD-L1). (G) Graphs show compiled data of CD8<sup>+</sup> T cell CFSE dilution from PBMC (left panel), Lymph node (middle panel), or Spleen (right panel) as percent of max (<i>n</i> = 4). P values were calculated by Wilcoxon matched-pairs signed ranked test.</p

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oai:figshare.com:article/1634597Last time updated on 2/12/2018

This paper was published in FigShare.

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