10.1371/journal.pone.0150197.g002

Plasma processing method comparison.

Abstract

<p>Whole blood was collected from 20 NSCLC patients and processed using various tube types, incubation times and centrifugation steps. A subset of these patients (n = 5) had whole blood processed to assess a single vs. double centrifugation step. DNA was measured using the ABI TaqMan® RNase P Detection Reagent Kit <b>A:</b> Blood was collected using EDTA blood collection tube (EDTA) and processed after 2 hr or 72 hr. <b>B:</b> Blood was collected using cell free DNA blood collection tube (cfDNA BCT) and processed after 2 hr or 72 hr. <b>C:</b> Blood was collected using EDTA or cfDNA BCT tubes and processed after 2 hr. <b>D:</b> Blood was collected using EDTA or cfDNA BCT tubes and processed after 72 hr. <b>E:</b> Blood collected in EDTA tubes after 2 hr incubation and processed using either single or double centrifugation. <b>F:</b> Blood collected in EDTA tubes after 72 hr incubation and processed using either single or double centrifugation. Results are displayed for each patient. Statistical analysis was performed using a paired Student’s t-test where; **p<0.01.</p

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oai:figshare.com:article/3022891Last time updated on 2/12/2018

This paper was published in FigShare.

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