Netrin-1 increases HCV through the UNC5A receptor.
Abstract<p>Huh7.5 cells were transfected with siRNA against each UNC5 receptor or with a nontargeting control siRNA and infected at a MOI of 0.1 24 h after seeding. Cells were then trypsinized at day five post-infection before undergoing a second siRNA transfection. Recombinant soluble Netrin-1-Fc was added to the medium 12 h after transfection. Intracellular HCV RNA was quantified by RT-qPCR at each time point (left-hand graphs; data are represented as mean ± standard deviation, <i>n</i> = 3, Wilcoxon test, <i>p</i> < 0.05), while siRNA-mediated knockdown was quantified for each <i>UNC5</i> transcript on days four and eight post-infection by RT-qPCR (right-hand graphs; data shown as mean ± standard deviation, <i>n</i> = 3). The underlying data for panels in this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002421#pbio.1002421.s001" target="_blank">S1 Data</a>.</p