Pathogenic <i>Chlamydia</i> Lack a Classical Sacculus but Synthesize a Narrow, Mid-cell Peptidoglycan Ring, Regulated by MreB, for Cell Division - Fig 8


<p><b>(a)</b> SIM of chlamydial inclusions in which cells were pulsed for two hours with 4 mM EDA—DA, after which the MreB polymerization inhibitor A22 (75 μM) was added to the medium. In the two far-right panels, medium containing A22 was removed after one hour. Cells were then washed, fresh medium without new EDA—DA was added, and cells were allowed to grow an additional 30 and 60 minutes, respectively. At indicated time points, cells were fixed, permeabilized, and fluorescently labeled via click chemistry and anti-<sub>CT</sub>MOMP. Scale bars = 1 μm. <b>(b)</b> Quantitative analysis of the mean fluorescence intensity of chlamydial inclusions initially pulsed with EDA-DA and subsequently chased under the times and conditions indicated. Fluorescence values were collected for ~250 inclusions per group, statistics were obtained utilizing an unpaired t test with Welch's correction and error bars represent standard deviations of the mean. Analysis was conducted in triplicate and is representative of two biological replicate experiments. <b>(c)</b> Graphical model representing the experimental approach and results from <b>(b)</b>.</p

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oai:figshare.com:article/3222589Last time updated on 2/12/2018

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