10.1371/journal.pone.0154702.g005

Identification of Ser-39 and Ser-40 in hCKβ as the major sites of PKA phosphorylation.

Abstract

<p>A) Schematic representation of potential sites of PKA phosphorylation in hCKβ as determined by mass spectrometry. <i>In vitro</i> phosphorylation of B) GST-hCKβ and GST-∆42NhCKβ recombinant fusion proteins, C) hCKβ, S39AhCKβ, S40AhCKβ, S42AhCKβ, S39A/S42AhCKβ, S40A/S42AhCKβ, and D) S39A/S40AhCKβ mutant proteins. The pictures are representative of at least two independent experiments. All CKβ mutant proteins were <i>in vitro</i> phosphorylated with 80 U PKA as described in the experimental procedures. Five micrograms of phosphorylated CKβ were loaded in each lane, and phosphorylation was detected with Pro-Q Diamond phosphoprotein stain. Total protein was stained with either Coomassie blue (B & D) or SYPRO<sup>®</sup> Ruby stain (C).</p

Similar works

Full text

thumbnail-image
oai:figshare.com:article/3308023Last time updated on 2/12/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.