Suppression of the mRNA decay phenotype of the <i>lsm1-27</i> mutant (C-terminal truncation mutant of <i>LSM1</i>) in <i>trans</i> by the various mutant versions of the C-terminal extension peptide, upon over expression.


<p>RNA isolated from <i>lsm1-27</i> cells expressing wild type or various mutant versions of the C-terminal extension peptide of Lsm1 from multi copy <i>2μ</i> vectors were subjected to Northern analysis to reveal the <i>MFA2pG</i> mRNA and the poly(G) fragments. The fractional contribution of the poly(G) fragments to the total signal (total = full-length mRNA + trimmed and normal poly(G) fragments) was approximated for each sample via quantitation using the phosphorimager and normalized to the value obtained for <i>lsm1-27</i> cells expressing wild type C-terminal extension peptide. Samples with approximate poly(G) fragment levels that are ≥ 80%, 60% to 80% and <60% of the value for cells expressing wild type peptide are marked with +++, ++ and + below the corresponding lanes in the figure.</p

Similar works

Full text

oai:figshare.com:article/3890949Last time updated on 2/12/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.