Abstract

<p>(A) The three DNA strand exchange containing 10 <b>μ</b>M nt M13mp18 cssDNA and 3.5 <b>μ</b>M HsRecA or EcRecA, were previously incubated per 20 min at 37°C, and then 3 <b>μ</b>M ATP and 1 <b>μ</b>M SSB were added and incubated for an additional 10 min. The minutes shown represents the time of reaction after addition of 20 <b>μ</b>M nt M13mp18 ldsDNA. (B) The percentage of duplex substrate converted into the nicked circular duplex (NC product) is plotted against the time.</p

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oai:figshare.com:article/3500999Last time updated on 2/12/2018

This paper was published in FigShare.

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