Effects of ATOR on cardiomyocyte apoptosis and the NF-κB pathway in diabetic hearts.


<p><b>(A).</b> Total protein extracted from heart tissues was subjected to western blot analysis for caspase-3 and cleaved caspase-3. <b>(B).</b> Heart tissues were sectioned at 3–4 μm and the slides were processed for TUNEL staining to detect apoptotic cells (arrows represent TUNEL-positive cells). <b>(C).</b> Total protein was extracted from heart tissues and IKK/IκBα expression detected by western blotting. <b>(D).</b> Cytoplasmic and nuclear proteins were extracted from heart tissues and nuclear translocation of NF-κB determined. <b>(E).</b> GSK-3β and PP2Ac phosphorylation was detected in total protein extracts from heart tissues. TUNEL assay images were obtained by microscopy with original magnification 400×.</p

Similar works

Full text

oai:figshare.com:article/4237106Last time updated on 2/12/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.