oaioai:cetus.saber.ula.ve:123456789/31405

Antimicrobial susceptibility of Staphylococcus aureus strains isolated from subclinical bovine mastitis milk and bulk tank milk

Abstract

Staphylococcus aureus es uno de los principales patógenos bacterianos de mastitis bovina en el estado Zulia - Venezuela. El objetivo del presente estudio fue determinar la susceptibilidad a los agentes antimicrobianos de cepas de S. aureus aisladas de muestras de leche de cuartos mamarios con mastitis subclínica y leche de tanque en tres fincas del estado Zulia. Un total de 88 cepas fueron analizadas, de estos aislamientos 81 fueron aislados de leche de cuartos mamarios y 7 de leche de tanque. A las cepas se le determinó la sensibilidad a los agentes antimicrobianos por el método de difusión del disco en agar, correspondientes a: vancomicina (Va), estreptomicina (S), clindamicina (Cc), eritromicina (E), tetraciclina (Te), penicilina (Pe), ciprofloxacina (Cip), cefoxitina (Fox), cloranfenicol (C), enrofloxacina (Enr), rifampicina (Rif), trimetoprim/sulfametoxazol (SXT), oxacilina (Ox) y gentamicina (G). La concentración inhibitoria mínima (CIM) se determinó a Pe, Va, Ox, y E. Las cepas de S. aureus aisladas de leche de cuartos mamarios fueron resistentes a Pe (12,3%), Enr (8,6%), Cip, S (4,9%, respectivamente), E (3,7%), Cc, y Rif (2,5%, respectivamente). Las cepas aisladas de leche de tanque fueron resistentes a Pe y Te (28,6%) y Cc (14, 3%). Los resultados indican que los aislamientos de S. aureus provenientes de leche de cuartos mamarios con mastitis subclínica fueron más susceptibles a todos los agentes antimicrobianos (70%) en comparación con las cepas procedentes de la leche de tanque (57%).367-376kutchynskaya@gmail.comBimestralStaphylococcus aureus is one of the main bacterial pathogens involved in bovine mastitis in Zulia State-Venezuela. The objective of this research was to determine the susceptibility to antimicrobial agents of Staphylococcus aureus isolated from the mammary quarter milk of subclinical mastitis cows and from bulk tank milk in three dairy farms of Zulia State. A total of 88 strains were analyzed, of these isolates 81 were isolated from quarter milk and 7 from bulk tank milk. The susceptibility to antimicrobial agents of those strains was determined by agar disk diffusion test. The antibiotics tested were: vancomycin (Va), streptomycin (S), clindamycin (Cc), erithromycin (E), tetracycline (Te), penicillin (Pe), ciprofloxacin (Cip), cefoxitin (Fox), chloramfenicol (C), enrofloxacin (Enr), rifampicin (Rif), trimethoprim/sulfametoxazole (SXT), oxacillin (Ox) and gentamicin. (G). Minimum Inhibitory Concentration (MIC) for Pe, Va, Ox and E was determined. S. aureus strains isolated from quarter milk were resistant to Pe (12.3%), Enr (8.6%), Cip, S (4.9%, respectively), E (3.7%), Cc, and Rif (2.5%, respectively). Strains isolated from bulk tank milk were resistant to Pe and Te (28.6%, respectively) and Cc (14.3%). The results indicate that isolates of S. aureus from mammary quarter milk of subclinical mastitis were more susceptible to all antimicrobial agents (70%) compared with strains from the bulk tank milk (57%)

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Repositorio Institucional de la Universidad de Los Andes

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oaioai:cetus.saber.ula.ve:123456789/31405Last time updated on 8/30/2013

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