<i>S</i>. <i>mansoni</i> infected rat sera recognize reduction-sensitive epitopes in recombinant insect cell extracts containing SmLy6 proteins.
<p>Insect cells engineered to express SmLy6A, SmLy6B, SmLy6C and SmLy6F were extracted in a mild detergent buffer and resolved by SDS-PAGE under non-reducing or reducing conditions, as indicated. Filters with insect cell extracts containing recombinant SmLy6A, B, C or F were probed by anti-FLAG (<b>A</b>) or rat infection sera (1:2000) (<b>B</b>). Detection employed HRP/anti-FLAG tag or HRP/anti-rat IgG. Numbers indicate the positions of migration of molecular mass markers (kDa).</p
Medicine, Microbiology, Cell Biology, Neuroscience, Biotechnology, Evolutionary Biology, Ecology, Immunology, Mental Health, Infectious Diseases, Virology, Computational Biology, Environmental Sciences not elsewhere classified, anti-tegumental antibody response, Schistosoma mansoni infection, Strong Antibody Response, Major Tegumental Membrane Proteins Schistosomiasis, mansoni infection sera antibodies, tegumental surface membrane proteins, schistosome antigens, Schistosoma mansoni Infection, schistosome tegumental membrane antigens, 6B, 6F, adult schistosomes, Schistosoma mansoni worm antigens, insect host cells, tegumental membrane proteins, schistosomiasis vaccine development
DOI identifier: 10.1371/journal.pntd.0005306.g003
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