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Study of cationic lipopolymer/DNA interaction.

By C. Facundo Temprana (4506838), M. Jimena Prieto (4506829), Daniela E. Igartúa (4506832), A. Lis Femia (4506835), M. Silvia Amor (4506841) and Silvia del Valle Alonso (529255)

Abstract

<p>Gel retardation assay for (a) DMPC:DC<sub>8,9</sub>PC:DOTAP (1:1:0.2), DMPC:DC<sub>8,9</sub>PC:SA (1:1:0.2), DMPC:DC<sub>8,9</sub>PC:MCL (1:1:0.2) and DMPC:DC<sub>8,9</sub>PC (1:1) mixtures incubated at 0:1, 6:1, 8:1, 10:1, 12:1, 14:1, 16:1 cationic lipopolymer or lipopolymer/pCH110 plasmid DNA ratios (mol of lipids: mol of base pairs) and (b) DMPC:DC<sub>8,9</sub>PC:DOTAP (1:1:0.2), DMPC:DC<sub>8,9</sub>PC:SA (1:1:0.2), DMPC:DC<sub>8,9</sub>PC:MCL (1:1:0.2) and DMPC:DC<sub>8,9</sub>PC (1:1) mixtures incubated at 0:1, 8:1, 16:1, 24:1, 30:1, 36:1, 42:1 cationic lipopolymer or lipopolymer/pDsRed plasmid DNA ratios (mol of lipids: mol of base pairs). All lanes were loaded with 1 μg of plasmid DNA.</p

Topics: Biophysics, Biochemistry, Medicine, Cell Biology, Molecular Biology, Biotechnology, Cancer, Infectious Diseases, Virology, Chemical Sciences not elsewhere classified, 166- day incubation period, DMPC, Vero cell lines, DOTAP, plasmid DNA, cationic lipopolymers, PC, cationic addition methodology, Cationic lipopolymers, CL, vivo gene therapy, SA, cationic lipids, cationic lipopolymer-based gene delivery system, DNA protection capacity, DC, serum DNAses degradation, blood cell hemolysis, MCL
Year: 2017
DOI identifier: 10.1371/journal.pone.0186194.g002
OAI identifier: oai:figshare.com:article/5490844
Provided by: FigShare
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