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Effect of ROS modulation on sirtuin activity.

By Arne Björn Potthast (4529689), Theresa Heuer (4529692), Simone Johanna Warneke (4529695) and Anibh Martin Das (4529686)

Abstract

<p>(A) Relative amount of reactive oxygen species (ROS) in COX-deficient cells compared to control cells (B) Detection of relative amount of ROS in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol) (C-D) Enzymatic activities of SIRT1 (C) and SIRT 3 (D) in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol) (E-F) Relative transcript levels of SIRT1 (E) and SIRT 3 (F) after treatment with a ROS scavenger (tocopherol) or ROS inducers (paraquat, diclophenac) in control fibroblasts (G-H) Relative protein amount of SIRT1 (G) and SIRT 3 (H) in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol); (n = 3; all data are shown in mean ± SD; * p < 0.05).</p

Topics: Biochemistry, Cell Biology, Genetics, Molecular Biology, Biotechnology, Science Policy, NAD, sirtuin levels, mitochondrial energy metabolism, analyse sirtuin levels, COX, mitochondrial cytochrome c-oxidase deficiency Background Sirtuins, mitochondrial sirtuins SIRT 3, IV, activators SRT 1720, patient cells
Year: 2017
DOI identifier: 10.1371/journal.pone.0186517.g006
OAI identifier: oai:figshare.com:article/5530375
Provided by: FigShare
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