Novel core-shell nanoparticles and their application in high-capacity immobilization of enzymes

Abstract

Novel core-shell nanoparticles consisting of poly(methyl methacrylate) (PMMA) cores coated with synthetic polymer and biopolymer (polyethyleneimine, chitosan, and casein) shells were synthesized via direct graft copolymerization of methyl methacrylate from hydrophilic polymers in the absence of surfactant. Average hydrodynamic diameters of the nanoparticles ranged from 163 to 263 nm. High-capacity (up to 530 mg/g) immobilizations of enzymes and high-activity retained percentage (Espe) (up to 90%) were achieved.Department of Applied Biology and Chemical Technolog