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The N-terminal Arg-rich region of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus Nef is involved in RNA binding

By Asier Echarri, María Eugenia González and Luís Carrasco

Abstract

Comparison of the amino acid sequences of human immunodeficiency virus (HIV) Nef protein and several RNA-binding proteins shows similarities in some regions of these proteins. Thus, poliovirus protein 2C, an RNA-binding protein, shares with Nef the sequence YXQQ...MDD...DXXD. In addition, both proteins contain an Arg-rich motif that, in the case of poliovirus 2C, is involved in RNA-binding activity. Moreover, the RNA-binding, anti-terminator N proteins of λ,φ21 and P22 phages show sequence similarities with HIV Nef at the Arg-rich motif. To assess the significance of this motif, native and deletion variants of Nef protein were assayed for RNA-binding activity. The N-terminal 35 amino acids of HIV-1 Nef that comprise the Arg-rich motif are sufficient for RNA binding. Point mutations engineered at the Arg-rich motif of HIV-1 Nef revealed that basic amino acid residues are essential for RNA-binding activity. The Nef proteins from HIV-2 and SIV can also interact with RNA, while the same proteins with the N-terminal Arg-rich domain truncated fail to interact with RNA. These findings indicate that all three Nef proteins from HIV-1, HIV-2 and simian immunodeficiency virus belong to the RNA-binding family of proteins. The three proteins contain an Arg-rich region at the N-terminus which is necessary to interact with RNA.Direccion General de Investigacirin Científica y Técnica project number PB94-0148 and the institutional grant to the CBM of Fundación Ramón Areces are acknowledged for their financial support. A. E. is a holder of a Gobierno Vasco.Peer Reviewe

Topics: Human immunodeficiency virus 1: Human immunodeficiency virus 2, Simian immunodeficiency virus, Nef, RNA-binding protein
Year: 2018
DOI identifier: 10.1111/j.1432-1033.1997.00038.x
OAI identifier: oai:digital.csic.es:10261/159038
Provided by: Digital.CSIC
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