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Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.

By S Lepage, P Dubois, T K Ghosh, B Joris, S Mahapatra, M Kundu, J Basu, P Chakrabarti, S T Cole, M Nguyen-Distèche and J M Ghuysen


The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity

Publisher: 'American Society for Microbiology'
Year: 2010
DOI identifier: 10.1128/jb.179.14.4627-4630.1997
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