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A Novel Method to Seal Intractable Air Leakage Utilizing Autologous Dermal Fibroblast Sheets Fabricated with Cell Sheet Engineering(The 21st COE Symposium on the Frontier Regenerative Medicine)

By  正人 神崎,  恭正 大貫,  雅之 大和 and  光夫 岡野


One of the most troublesome complications is intraoperative or prolonged air leakage from the lung. Advances in surgical techniques, including the use of stapling devices, have minimized but not abolished such complications. Lung parenchymal defects are difficult to suture often both damages the normal lung and fails to stop the air leakage. Current methods including the use of various sealants, are ineffective in the closure of intraoperative air leaks, resulting in a increased patient morbidity, delayed removal of thoracic tubes, and prolonged hospitalization. We present the development of a novel lung air leak sealant using tissue engineered cell sheets. Cell sheets immediately and permanently seal air leaks in a dynamic function that allows for the extensive tissue contraction and expansion involved in respiration, with no postoperative recurrences. Moreover, we demonstrate that host mesothelial cells can migrate to cover the transplanted cells sheets, thereby confirming excellent biocompatibility and integration with host tissues. Finally, we show the use of skin fibroblasts as an effective autologous cell source that can be easily applied. This study shows for the first time, the development of an immediate and permanent lung air leak sealant, suitable for future clinical applications

Topics: air leak, cell sheet, fibroblast, autologous cell, sealant
Publisher: 東京女子医科大学学会
Year: 2006
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