Article thumbnail

Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia

By Daniel MARTINS-DE-SOUZA, Wagner F. GATTAZ, Andrea SCHMITT, Giuseppina MACCARRONE, Eva HUNYADI-GULYAS, Marcos N. EBERLIN, Gustavo H. M. F. SOUZA, Sergio MARANGONI, Jose C. NOVELLO, Christoph W. TURCK and Emmanuel DIAS-NETO

Abstract

Schizophrenia is likely to be a consequence of serial alterations in a number of genes that, together with environmental factors, will lead to the establishment of the illness. The dorsolateral prefrontal cortex (Brodmann`s Area 46) is implicated in schizophrenia and executes high functions such as working memory, differentiation of conflicting thoughts, determination of right and wrong concepts, correct social behavior and personality expression. We performed a comparative proteome analysis using two-dimensional gel electrophoresis of pools from 9 schizophrenia and 7 healthy control patients` dorsolateral prefrontal cortex aiming to identify, by mass spectrometry, alterations in protein expression that could be related to the disease. In schizophrenia-derived samples, our analysis revealed 10 downregulated and 14 upregulated proteins. These included alterations previously implicated in schizophrenia, such as oligodendrocyte-related proteins (myelin basic protein and transferrin), as well as malate dehydrogenase, aconitase, ATP synthase subunits and cytoskeleton-related proteins. Also, six new putative disease markers were identified, including energy metabolism, cytoskeleton and cell signaling proteins. Our data not only reinforces the involvement of proteins previously implicated in schizophrenia, but also suggests new markers, providing further information to foster the comprehension of this important disease. (C) 2008 Elsevier Ltd. All rights reserved

Topics: Schizophrenia, Proteomics, Mitochondria, Cytoskeleton, Myelinization, Dorsolateral prefrontal cortex, ANTERIOR CINGULATE CORTEX, FIBRILLARY ACIDIC PROTEIN, GENE-EXPRESSION DIFFERENCES, MAJOR DEPRESSIVE DISORDER, MYELINATION-RELATED GENES, HEAT-SHOCK PROTEINS, CEREBROSPINAL-FLUID, BIPOLAR DISORDER, WHITE-MATTER, OXIDATIVE STRESS, Psychiatry
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Year: 2009
DOI identifier: 10.1016/j.jpsychires.2008.11.006
OAI identifier: oai:www.producao.usp.br:BDPI/31510
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://dx.doi.org/10.1016/j.jp... (external link)
  • http://producao.usp.br/handle/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.