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RNA Synthesis in the Tracheal Epithelial Cells of Aging Mice as Revealed by Electron Microscopic Radioautography

By Int J Cur Sci Res Tetsuji Nagata

Abstract

For the purpose of studying the aging changes of macromolecular synthesis in the tracheal epithelial cells of experimental animals, we studied 10 groups of aging mice during development and aging from fetal day 19 to postnatal month 24. They were injected with 3H-uridine, a precursor for RNA synthesis, sacrificed and the tracheal tissues were taken out, fixed and processed for light and electron microscopic radioautography. On many radioautograms the localization of silver grains demonstrating RNA synthesis in tracheal epithelial cells in respective aging groups were analyzed qualitatively and quantitatively. The number of mitochondria per cell, the number of labeled mitochondria with silver grains and the number of silver grains in each cell in respective aging groups were analyzed quantitatively in relation to the aging of animals. The results revealed that the RNA synthetic activity as expressed by the incorporations of RNA precursor, i.e., the number of silver grains in cell nuclei, cell organelles, changed due to the aging of animals. The number of mitochondria, the number of labeled mitochondria and the mitochondrial labeling index labeled with silver grains were counted in each tracheal epithelial cell. It was demonstrated that the number of mitochondria increased from embryonic day 19 to postnatal newborn day 1, 3, 9, 14, adult month 1, 2, reaching the maximum and kept plateau, while the number of labeled mitochondria increased from embryonic day 19 to postnatal adult month 2, reaching the maximum but the labeling indices showing RNA synthesis increased from embryonic day 19 to postnatal newborn day 14 and adult month 1 and decreased to year 2, indicating the aging changes. Based upon our findings, available literature on macromolecular synthesis in mitochondria of various cells are reviewed

Topics: JOURNALS
Publisher: CurrentSciDirect Publications
Year: 2011
OAI identifier: oai:cogprints.org:7303
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    1. (1996). A light and electron microscopic radioautographic study on RNA synthesis in the retina of chick embryo. doi
    2. (1969). A new technique for electron microscopic radioautography of soluble compouds. doi
    3. Age related changes of DNA repair and mitochondrial DNA synthesis in the mouse brain. doi
    4. Aging changes of DNA synthesis in the submandibular glands of mice as observed by light and electron microscopic radioautography.
    5. (2006). Aging changes of macromolecular synthesis in the avian and mammalian eyes as revealed by microscopic radioautography. Ann Rev Biomed Sci. doi
    6. Cell-type-specific differences in age-related changes of DNA repair in the mouse brain - Molecular basis for a new approach to understand the selective neuronal vulnerability in Alzheimer's disease.
    7. Demonstration of extranuclear nucleic acid synthesis in mammalian cells under experimental conditions by electron microscopic radioautography.
    8. (1997). Development and DNA synthesis in the retina of chick embryo observed by light and electron microscopic radioautography. Cell Mol Biol.
    9. DNA synthesis in the pancreatic acinar cells of aging mice as revealed by electron microscopic radioautography. IIOABJournal . 2011b; 1: doi
    10. Electron microscopic radioatuographic studies on macromolecular synthesis in mitochondria of various cells.
    11. (2007). Electron Microscopic Radioautographic doi
    12. Electron microscopic radioautographic studies on macromoleclular synthesis in mitochondria of some organs in aging animals.Bull.
    13. Electron microscopic radioautographic studies on macromolecular synthesis in mitochondria of animal cells in aging. Ann Rev Biomed Sci. doi
    14. Electron microscopic radioautographic studies on macromolecular synthesis in mitochondria ofanimal cells in aging. Ann Rev Biomed Sci. doi
    15. Electron microscopic radioautographic study of DNA synthesis in the livers of aging mice. doi
    16. (1996). Electron microscopic radioautographic study on DNA synthesis and the ultrastructure of the adrenal gland in aging mice. Med Electron Microsc. doi
    17. Electron microscopic radioautographic study on DNA synthesis of mitochondria in adrenal medullary cells of aging mice.
    18. Electron microscopic radioautographic study on macromolecular synthesis in hepatocyte mitochondria of aging mouse. doi
    19. (2008). Electron microscopic radioautographic study on mitochondrial DNA synthesis in adrenal cortical cells of developing and aging mice. The Sci World J.
    20. Electron microscopic radioautographic study on mitochondrial DNA synthesis in adrenal cortical cells of developing mice.
    21. (2008). Electron microscopic radioautographic study on mitochondrial DNA synthesis in adrenal cortical cells of developing mice. J Cell Tis Res. doi
    22. Electron microscopic radioautographic study on mitochondrial DNA synthesis in adrenal medullary cells of developing and aging mice.
    23. Electron microscopic radioautographic study on mitochondrial DNA synthesis in adreno-cortical cells of aging ddY mice.Bull.
    24. (2005). Electron microscopic radioautographic study on mitochondrial DNA synthesis in hepatocytes of aging mouse. Ann Microsc. doi
    25. Electron microscopic radioautographic study on mitochondrial RNA synthesis in adrenocortical cells of aging mice.
    26. (1994). Electron microscopic radioautographic study on nucleic acid synthesis in perinatal mouse kidney tissue. Med Electron Microsc. doi
    27. (1994). Electron microscopic radioautographic study on nucleic acid synthesis of perinatal mouse retina. doi
    28. Electron microscopic radioautographic study on nucleic acids synthesis in hepatocyte mitochondria of developing mice.Trends Cell Mol.Biol.
    29. (2006). Electron microscopic radioautographic study on protein synthesis in hepatocyte mitochondria of developing mice. Ann Microsc. doi
    30. (2007). Electron microscopic radioautographic study on protein synthesis in mitochondria of binucleatehepatocytes in aging mice. The Scientific World J. doi
    31. (1994). Electron microscopic radioautographic study on the nucleic acid and protein synthesis in the aging mouse testis. doi
    32. (1994). Electron microscopic radioautographic study on the synthesis of DNA, RNA and protein in the livers of aging mice. doi
    33. (1986). Electron microscopic radioautography of nucleic acid synthesis in pancreatic acinar cells of prenatal and postnatal aging mice. doi
    34. (1963). Intramitochondrial fibers with DNA characteristics. I. Fixation and electron staining reactions. II. Enzymatic and other hydrolytic treatments.
    35. (2003). Light and electron microscopic radioautographic studies on macromolecular synthesis in amitotic hepatocytes of aging mice. Cell Mol Biol. doi
    36. (1999). Light and electron microscopic radioautographic studies on RNA synthesis in aging mouse adrenal gland. Acta Anat Nippon.
    37. (1993). Light and electron microscopic radioautographic studies on the RNA synthesis of peri-implanting pregnant mouse uterus during activation of receptivity for blastocyst implantation. Cell Mol Biol.
    38. (1992). Light and electron microscopic radioautography of DNA synthesis in the endometria of pregnant ovariectomized mice during activation of implantation window. Cell Mol Biol.
    39. (1979). Light and electron microscopic radioautography of nucleic acid synthesis in mitochondria and peroxisomes of rat hepatic cells during and after
    40. (1998). Mitochondrial DNA synt hesis studied autoradiographically in various cell types in vivo. doi
    41. (1982). Nucleic acid synthesis in proliferating p e r o x i s o m e s o f r a t l i v e r a s r e v e a l e d b y e l e c t r o n microscopicalradioautography.Histochem J. doi
    42. Protein synthesis in hepatocytes of mice as revealed by electron microscopic radioautography.In, Protein Biosynthesis.Esterhouse,
    43. (1996). Radioautographic studies on aging changes of DNA synthesis and the ultrastructural development of mouse adrenal gland. Cell Mol Biol.
    44. (1984). Radioautographic studies on the nucleic acid synthesis in the r e t i n a o f c h i c k e n e m b r y o I I . E l e c t r o n m i c r o s c o p i c radioautography.Shinshu Med
    45. Radioautographic study of the macromolecular synthesis of Leydig cells in aging mouse testis.
    46. Recent studies on macromolecular synthesis labeled with 3Hthymidine in various organs as revealed by electron microscopic radioautography. Cur Radiopharm. doi
    47. RNA synthesis in the pancreatic acinar cells of aging mice as revealed by electron microscopic radioautography. J Pancreas. 2011c; 12: doi
    48. Studies on DNA synthesis of aging mice by means of electron microscopic radioautography.
    49. (1996). Study on DNA synthesis of cellular elements in the cerebella of aging mice by light and electron microscopic radioautography.
    50. (1997). Unscheduled DNA synthesis and mitochondrial DNA synthetic rate following injuring of the facial nerve. ActaNeuropathol. doi

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