The first example of thin layer electrochemistry coupled to epifluorescence microscopy in the total internal
reflectance mode is described and applied to the investigation of electrochemically modulated fluorescence of
an organic dye (chloromethoxytetrazine) in solution. This technique allows to generate full redox switch of
fluorescence when converting reversibly the dye into its anion radical, as well as to record the spectral
features of the electrogenerated species. Recording simultaneously fluorescence intensity and lifetime along
with coulombic charge as a function of the electrode potential will lead to a deep insight into the redox
quenching mechanism
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