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Coupling thin layer electrochemistry with epifluorescence microscopy: an expedient way of investigating electrofluorochromism of organic dyes

By F. Miomandre, C. Allain, G. Clavier, J-F. Audibert, R. B. Pansu, P. Audebert and Frantisek Hartl


The first example of thin layer electrochemistry coupled to epifluorescence microscopy in the total internal\ud reflectance mode is described and applied to the investigation of electrochemically modulated fluorescence of\ud an organic dye (chloromethoxytetrazine) in solution. This technique allows to generate full redox switch of\ud fluorescence when converting reversibly the dye into its anion radical, as well as to record the spectral\ud features of the electrogenerated species. Recording simultaneously fluorescence intensity and lifetime along\ud with coulombic charge as a function of the electrode potential will lead to a deep insight into the redox\ud quenching mechanism

Publisher: Elsevier
Year: 2011
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