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Murine genotype impacts pancreatitis severity and systemic inflammation: An experimental study

By Gabriel J. Seifert, Karoline C. Sander, Sabine Richter and Uwe A. Wittel

Abstract

Background: Little is known regarding the impact of host response in acute pancreatitis. Here, we induce murine necrotizing pancreatitis in 9 different mouse strains. Materials and methods: We examined 9 different mouse strains: Balb/CB4J, C3H/HEJ, NOD/SHILT, A/J, AKR/J, C57BI/6J, DBA/2J, FVB/NJ, 129S1/SvlmJ. 10 animals per strain were randomly allotted to two groups. Sterile necrotizing pancreatitis was induced by injection of taurocholate into the common bile duct. Control animals were injected with saline. Every 6 h, clinical parameters were examined and scored. After 24 h, animals were sacrificed to examine and compare serum enzymes, histology, bronchoalveolar lavage fluid, and serum IL-6. Results: Histologically, taurocholate treated animals scored significantly higher than control animals. Concordantly, serum lipase and amylase were significantly elevated in pancreatitis animals in all strains. NOD/SHILT and AKR/J mice had the highest enzyme activity. 24 h after induction, there were no signs of increased pulmonary vascular leak in taurocholate animals. Remarkably, interleukin 6 was not increased at all in C57BL/6J, C3H/HeJ, and 129S1/SvlmJ mice compared to all other strains. Conclusion: The genetic strain has an impact on pancreatitis severity and systemic inflammatory response in a murine taurocholate induction model. Analogous differences in humans may partially account for the disparity in post-ERCP pancreatitis

Topics: Genetic background, Genetic strain, Murine pancreatitis, Taurocholate, Severe acute pancreatitis, Medicine (General), R5-920
Publisher: Elsevier
Year: 2017
DOI identifier: 10.1016/j.amsu.2017.09.012
OAI identifier: oai:doaj.org/article:7304a49bb835455097c152db7c596e53
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