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Decrease in GLUT4 protein in adipose tissue of rats treated with subcutaneous injections of soybean or sunflower oils.

By Paula Eichler

Abstract

Tratamos ratos por 7 dias com injeções subcutâneas de óleos de soja (S) ou de girassol (G). Verificamos o desenvolvimento de resistência insulínica, com diminuição de GLUT4 e de sua translocação no tecido adiposo de ratos tratados com estes óleos, apesar de aumento no RNAm de GLUT4 no músculo e no tecido adiposo. O tratamento com girassol diminuiu RNAm de GLUT1 no adiposo, onde também diminuiu a quantidade de RNAm de NF-kB. (outros fatores transcricionais não apresentaram alteração: PPARg, MEF2A e MF2D). Dosamos ácidos graxos no plasma, fígado (sem alterações), músculo (palmitoléico aumentou no grupo G; linolênico diminuiu no S e G e araquidônico aumentou no S) e adiposo (palmítico e esteárico aumentaram e linoleico diminuiu nos grupos S e G, além de aumentar a proporção saturados/ insaturados).We treated rats for 7 days with subcutaneous soybean (SB) oil or sunflower (SF)oil injections. Insulin resistance was developed, with a decrease in GLUT4 quantity and translocation in adipose tissue in the rats treated with those oils, despite GLUT4 mRNA increase in muscle and adipose tissue. Sunflower treatment led to decrease in GLUT1mRNA in adipose tissue, where NF- kB mRNA was also decreased (other transcriptional factors did not change: PPARg, MEF2A e MF2D). Fatty acids were measured in the plasma, the liver (no changes), muscle (palmitoleic increased in SF, linolenic decreased in SB and SF and arachidonic increased in SB) and adipose tissue (palmitic and stearic increased and linoleico decreased in SB and SF, besides increasing in saturated/ unsaturated ratio

Topics: Biologia, Metabolismo energético, Metabolismo de carbohidrato, Metabolismo, Fisiologia, Metabolism, Energetic metabolism, Carbohidrate metabolism, Biology, Physiology
Publisher: 'Universidade de Sao Paulo, Agencia USP de Gestao da Informacao Academica (AGUIA)'
Year: 2008
DOI identifier: 10.11606/T.42.2008.tde-16092008-115901
OAI identifier: oai:teses.usp.br:tde-16092008-115901
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