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An OR logic gate based on two molecular beacons

By Jing Guo and Renqiang Yang

Abstract

<div style="text-align: left; widows: 2; text-transform: none; background-color: #ffffff; text-indent: 0px; letter-spacing: normal; font: 200 12px Helvetica, Arial, Verdana, sans-serif; white-space: normal; orphans: 2; color: #58595b; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><div id="highlighting-container" class="disease_highlightoff habitat_highlightoff place_highlightoff organism_highlightoff taxon_highlightoff institution_highlightoff date_highlightoff person_highlightoff citationtype_highlightoff chemical_highlightoff species_highlightoff protein_highlightoff"><table border="0" style="margin: 1em 0px 0px"><tbody><tr><td class="indented_text" style="font-size: 10pt">Design of elementary molecular logic gates is the key and the fundamental of performing complicated Boolean calculations. Herein, we report a strategy for constructing a DNA-based OR gate by using the mechanism of sequence recognition and the principle of fluorescence resonance energy transfer (FRET). In this system, the gate is entirely composed of a single strand of DNA (A, B and C) and the inputs are the molecular beacon probes (<span class="protein"><a style="color: #000000; font-weight: normal; text-decoration: none" href="http://europepmc.org/abstract/MED/22278176/?whatizit_url_gene_protein=http://www.uniprot.org/uniprot/?query=MB1&amp;sort=score" target="_blank">MB1</a></span><span class="Apple-converted-space">&nbsp;</span>and<span class="Apple-converted-space">&nbsp;</span><span class="protein"><a style="color: #000000; font-weight: normal; text-decoration: none" href="http://europepmc.org/abstract/MED/22278176/?whatizit_url_gene_protein=http://www.uniprot.org/uniprot/?query=MB2&amp;sort=score" target="_blank">MB2</a></span>). Changes in fluorescence intensity confirm the realization of the OR logic operation and electrophoresis experiments verify these results. Our successful application of DNA to perform the binary operation represents that DNA can serve as an efficient biomaterial for designing molecular logic gates and devices.</td></tr></tbody></table></div></div><div class="publisherLink" style="text-align: right; widows: 2; text-transform: none; background-color: #ffffff; text-indent: 0px; margin: 20px 0px 0px; letter-spacing: normal; font: 200 12px Helvetica, Arial, Verdana, sans-serif; white-space: normal; orphans: 2; color: #58595b; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><a style="color: #20699c; text-decoration: none" href="http://europepmc.org/abstract/MED/22278176/abstract/MED/22278176?europe_pmc_extredirect=http://dx.doi.org/10.1039/c2mb05477a" class="abs_publisher_link" onclick="var w = window.open(href, '', 'scrollbars=yes,location=no,menuBar=no,resizable=yes,status=no,toolbar=no,width=900,height=900'); if(w.blur) w.focus(); return false;"><span>Read Article at<span class="Apple-converted-space">&nbsp;</span><strong>ww</strong></span></a></div>Design of elementary molecular logic gates is the key and the fundamental of performing complicated Boolean calculations. Herein, we report a strategy for constructing a DNA-based OR gate by using the mechanism of sequence recognition and the principle of fluorescence resonance energy transfer (FRET). In this system, the gate is entirely composed of a single strand of DNA (A, B and C) and the inputs are the molecular beacon probes (MB1 and MB2). Changes in fluorescence intensity confirm the realization of the OR logic operation and electrophoresis experiments verify these results. Our successful application of DNA to perform the binary operation represents that DNA can serve as an efficient biomaterial for designing molecular logic gates and devices

Topics: 生物材料::先进功能有机材料, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biochemistry & Molecular Biology, DNA LOGIC, CHEMICAL INPUTS, COMPUTATION, CIRCUITS, QUADRUPLEX, PROTEINS, BINDING, DESIGN, ENZYME, PH
Year: 2012
DOI identifier: 10.1039/c2mb05477a
OAI identifier: oai:ir.qibebt.ac.cn:337004/1191
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