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A new paradigm for MAPK: Structural interactions of hERK1 with mitochondria in HeLa cells

By S.</div> <div class="autor_fcen" id="3432">Galli, O. Jahn, R. Hitt, D. Hesse, L. Opitz, U. Plessmann, H. Urlaub, J.J. Poderoso, E.A.</div> <div class="autor_fcen" id="4466">Jares-Erijman and T.M. Jovin

Abstract

Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct phosphorylation of downstream effectors located throughout the cell. In this study we determined that hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus. In the mitochondrial environment, hERK1 physically associates with (i) at least 5 mitochondrial proteins with functions related to transport (i.e. VDAC1), signalling, and metabolism; (ii) histones H2A and H4; and (iii) other cytosolic proteins. This work indicates for the first time the presence of diverse ERK-complexes in mitochondria and thus provides a new perspective for assessing the functions of ERK1 in the regulation of cellular signalling and trafficking in HeLa cells. © 2009 Galli et al.Fil:Galli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Topics: histone H2A, histone H4, mitogen activated protein kinase 1, mitogen activated protein kinase 3, voltage dependent anion channel, glutathione transferase, article, complex formation, controlled study, enzyme activity, gene control, gene expression, gene location, HeLa cell, human, human cell, mitochondrion, nucleotide sequence, protein protein interaction, protein transport, signal transduction, amino acid sequence, cell proliferation, gene expression profiling, gene expression regulation, mass spectrometry, metabolism, methodology, molecular genetics, proteomics, sequence homology, Amino Acid Sequence, Cell Proliferation, Gene Expression Profiling, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Glutathione Transferase, Hela Cells, Humans, MAP Kinase Signaling System, Mitochondria, Mitogen-Activated Protein Kinase 3, Molecular Sequence Data, Proteomics, Sequence Homology, Amino Acid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Year: 2009
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