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Acute diarrhea associated with Salmonella enterica in Belo Horizonte-MG: prevalence and characterization of isolates

By Mireille Ângela Bernardes Sousa, Edilberto Nogueira Mendes, Francisco José Penna, Luciano Amedée Péret-Filho and Paula Prazeres Magalhães

Abstract

Introduction: Acute infectious diarrhea is still regarded as a public health problem associated with a wide range of etiologic agents, from which Salmonella enterica is particularly worth mentioning inasmuch as it is a major cause of inflammatory diarrhea in both developed and developing countries. Objective: To assess the distribution of S. enterica among children with acute diarrhea in Belo Horizonte and to characterize bacterium isolates. Material and methods: The study group comprised a total of 157 children from low socioeconomic background. Stool samples were collected for leukocyte analysis and Salmonella bacterial culture. The isolates were serotyped and evaluated as to antimicrobial susceptibility profile, extended-spectrum β-lactamases (ESBL) production, and presence of virulence markers (invA, iroB, and spvC). RESULTS: A total of 5/3.2% children were infected by S. enterica, 3/60% by S. enterica Typhimurium, 1/20% by S. enterica Enteritidis and 1/20% S. enterica subsp. enterica serotype 8.20:z4,z23:-. Fecal leucocytes were detected in two out of five fecal specimens positive for S. enterica. Isolates from three children were resistant to nalidixic acid, nalidixic acid + chloramphenicol, and nalidixic acid + chloramphenicol + ampicillin. ESBL production was not detected. All samples presented invA and iroB genes. spvC marker was observed in isolates from two children infected by S. Typhimurium and S. Enteritidis. Conclusion: The results demonstrate that S. enterica infection is uncommon among children from our region. Furthermore, they indicate the need for periodic monitoring of bacterial antimicrobial susceptibility profile in order to establish suitable antimicrobial therapy when required

Topics: Salmonella enterica, acute diarrhea, salmonellosis, antimicrobial susceptibility, epidemiology
Publisher: Sociedade Brasileira de Citopatologia
Year: 2013
DOI identifier: 10.1590/s1676-24442013000100005
OAI identifier: oai:periodicos.ibict.br.JornalBrasileirodePatologiaeMedicinaLaboratorial:oai:scielo:S1676-24442013000100005
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