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Detection of Salmonella typhimurium using an electrochemical immunosensor.

By Faridah Salam and Ibtisam E. Tothill

Abstract

An electrochemical immunosensor based on screen-printed gold working electrode with onboard carbon counter and silver–silver chloride pseudo-reference electrode for Salmonella typhimurium detection is described in this paper. Monoclonal anti-S. typhimurium antibody was immobilized using physical and covalent immobilization via amine coupling of carboxymethyldextran on the surface of the gold working electrode. A direct sandwich enzyme-linked immunosorbent assays (ELISA) format was then developed and optimized using a polyclonal anti-Salmonella antibodies conjugated to horseradish peroxidase (HRP) as the enzyme label. 3,3′,5,5′-Tetramethylbenzidine dihydrochloride (TMB)/H2O2 was used as the enzyme mediator/substrate system. Electrochemical detection was conducted using chronoamperometry at −200 mV vs. onboard screen-printed Ag–AgCl pseudo-reference electrode. The applied potential was selected through the study of the electrochemical behaviour of bare gold electrode with TMB–H2O2–IgG–HRP system. S. typhimurium detection of 5 × 103 cells ml−1 and 20 cells ml−1 was achieved respectively for physical and covalent antibody immobilization. The developed sensor was then compared to a commercial ELISA kit and a chromogenic agar plating method for meat samples analysis. The sensor format shows a promising technology for simple and sensitive detection system for Salmonella contamination. Rapid detection of Salmonella is a key to the prevention and identification of problems relate

Topics: Immunosensor, Screen-printed gold electrode, Salmonella, Electrochemical detection, Food monitoring
Publisher: Elsevier Science B.V., Amsterdam.
Year: 2009
DOI identifier: 10.1016/j.bios.2009.01.025
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/3396
Provided by: Cranfield CERES
Journal:

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Citations

  1. (1985). Practice and Theory of Immunoassay,
  2. (2003). Rapid and on-line Instrumentation for Food Quality Assurance, Ibtisam E. Tothill (Editor). doi
  3. (2003). Rapid and on-line Instrumentation for Food Quality Assurance, Ibtisam E. Tothill (Editor). Woodhead Publishing Limited, doi
  4. (2008). Talanta, 74, 1621–1629.Page 20 of 28 Accepted Manuscript Schematic Diagram Gold working screen printed electrode TMBred
  5. (2006). Water Contamination Emergencies: Enhancing Our Response.

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