Skip to main content
Article thumbnail
Location of Repository

Use of volatile fingerprints for rapid screening of antifungal agents for efficacy against dermatophyte Trichophyton species

By Kamran Naraghi, Natasha Sahgal, Beverley Adriaans, Hugh Barr and Naresh Magan

Abstract

The potential of using an electronic nose (E-nose) as a rapid technique for screening the responses of dermatophytes to antifungal agents was studied. In vitro, the 50% and 90% effective concentration (EC) values of five antifungal agents including fungicides and antioxidant mixtures against Trichophyton rubrum and Trichophyton mentagrophytes were obtained by mycelial growth assays. The qualitative volatile production patterns of the growth responses of these fungi to the EC values incorporated into solid media were analysed after 96-120h incubation at 25°C using headspace analyses using five replicates per treatment. Overall, results, using principal components analysis and cluster analysis, demonstrated that it was possible to differentiate between various treatments within 96-120h of growth. The EC50 values were discriminated from the controls while the EC90 concentration treatments were often grouped with the agar blanks because of very slow growth. This study showed that potential exists for using qualitative volatile patterns as a rapid screening method for antifungal agents against micro-organisms. This approach could significantly improve and facilitate the monitoring of antimicrobial drug activities and infection control programmes and perhaps also for monitoring of drug resistance buildup in microbial population

Publisher: Elsevier Science B.V., Amsterdam.
Year: 2010
DOI identifier: 10.1016/j.snb.2009.12.031
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/5729
Provided by: Cranfield CERES
Journal:

Suggested articles

Citations

  1. (2005). Antifungal susceptibilities of dermatophytic agents isolated from clinical specimens,
  2. (2001). Antifungal Susceptibility Testing: Practical Aspects and Current Challenges. doi
  3. (2004). Chapter 31: Mycology. In: Rook’s Textbook of Dermatology, 7 th ed doi
  4. Clinical evaluation of the electronic nose in the diagnosis of ear, nose and throat infection: a preliminary study. doi
  5. (2004). Detection of Mycobacterium tuberculosis (TB) in vitro and in situ using an electronic nose in combination with a neural network system. doi
  6. (2004). Electronic noses and disease diagnostics. doi
  7. (2006). Fast identification of ten clinically important micro-organisms using an electronic nose. doi
  8. (2008). Fungal volatile fingerprints: discrimination between dermatophyte species and strains by means of an electronic nose. Sensors and Actuators doi
  9. (2002). Identification of upper respiratory bacterial pathogens with the electronic nose, doi
  10. (2007). In vitro discrimination of tumor cell lines with an electronic nose, Otolaryngology -Head and Neck Surgery 137 doi
  11. (2006). In vitro methods for antifungal susceptibility testing of Trichophyton species. doi
  12. Medical Mycology. In: doi
  13. (2006). Prospects for clinical application of electronic-nose technology to early detection of Mycobacterium tuberculosis in culture and sputum. doi
  14. (2005). Real-time detection of common microbial volatile organic compounds from medically important 15 fungi by Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS), doi
  15. (2006). Tinea capitis in a paediatric population,
  16. (2006). Trichophyton species: use of volatile fingerprints for rapid identification and discrimination. doi
  17. (2002). Use of an electronic nose system for diagnoses of urinary tract infections, doi
  18. (2000). Volatiles as an indicator of fungal activity and differentiation between species, and the potential use of electronic nose technology for early detection of grain spoilage, doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.