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Biomimetic sensors for HbA1c

By Anna Biela

Abstract

Diabetes mellitus is a growing health problem worldwide. Suitable long-term control and management of this disease are enabled by determination of glycated haemoglobin (HbA1c) in blood. The results are given as %HbA1c of total haemoglobin. Presently available tests vary in cost and convenience and there is an identified need to introduce improved equipment for self-monitoring. This dissertation focuses on fast and straightforward detection of glycated haemoglobin (HbA1c) using cyclic voltammetry and chronoamperometry. Haemoglobin was determined by monitoring its reaction with potassium ferricyanide on screen printed electrodes at an oxidative potential +500 mV. A working electrode was modified with carbon nanotubes to enhance electron transfer. A calibration curve was linear in a range from 0.83 to 83 mg/mL. Another innovative approach to detecting haemoglobin using its enzymatic activity was also developed. Detection of haemoglobin was performed with hydroquinone and hydrogen peroxide on screen printed electrodes at a potential -400 mV in a Flow Injection Analysis system (FIA). Cont/d

Publisher: Cranfield University
Year: 2010
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/6275
Provided by: Cranfield CERES

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Citations

  1. (1991). A first course in electrode processes. The Electrochemical Consultancy,
  2. (2005). A method for the molecular imprinting of hemoglobin on silica surfaces using silanes. doi
  3. (1992). A new kind of redox sensor based on conducting polymer films. doi
  4. (2006). A novel amperometric hydrogen peroxide biosensor based on immobilization of hemoglobin in linoleic acid monolayer by Langmuir-Blodgett technique. Colloids and Surfaces A: Physicochemical and Engineering Aspects doi
  5. (2001). A reagentless glucose biosensor based on glucose oxidase entrapped into osmium-complex modified polypyrrole films. Sensors and Actuators, doi
  6. (1998). ABC of diabetes. doi
  7. (1996). Adsorption behaviour of serum albumin on electrode surfaces and the effects of electrode potential. doi
  8. (2006). Adsorption study of heme proteins on SBA-15 mesoporous silica with pore-filling models. doi
  9. (1985). Affinity chromatography a practical approach. doi
  10. (2007). Amperometric biosensor based on direct electrochemistry of hemoglobin in poly-allylamine (PAA) film. Thin Solid Films, doi
  11. (2001). Amperometric hydrogen peroxide biosensor with silica solgel/chitosan film as immobilization matrix. doi
  12. (2006). Amperometric protein sensor – fabricated as a polypyrrole, poly-aminophenylboronic acid bilayer. doi
  13. (2007). Amperometric third-generation hydrogen peroxide biosensor based on immobilization of Hb on gold nanoparticles/cysteine/poly(p-aminobenzene sulfonic acid)-modified platinum disk electrode. Colloids and Surfaces A: Physicochemical and Engineering Aspects, doi
  14. (2005). An efficient preparation of polyanionic affinity agent and its evaluation for the measurement of glycated hemoglobin. doi
  15. (2006). An immunosensor for haemoglobin based on impedimetric properties of a new mixed self-assembled monolayer. doi
  16. (2004). An investigation into outer-surface biofouling and electrode passivation effects on gold electrodes modified with Calix[4]resorcinarenetetrathiol and a PEG derivative on exposure to whole human blood. doi
  17. (2007). Analysis of blood proteins on protein arrays with a spectral surface plasmon resonance biosensor. doi
  18. (2003). Analytical chemistry. doi
  19. (2006). Analytical electrochemistry, 3 rd ed. doi
  20. Angewandte Chemie - doi
  21. (2006). Application of nanoparticles in electrochemical sensors and biosensors. doi
  22. (2005). Bioelectrochemically functional nanohybrids through co-assembling of proteins and surfactants onto carbon nanotubes: facilitated electron transfer of assembled proteins with enhanced faradic response. doi
  23. (2002). Biomedical diagnostic science and technology. doi
  24. (2000). Biosensor for environmental monitoring. The Netherlands: Harwood academic publishers. doi
  25. (1996). Biosensoring of hydrogen peroxide using new methylene blue N incorporated in a montmorillonite-modified horseradish peroxidase immobilization matrix as an electron shuttle. doi
  26. (2006). Biosensors and bioelectrochemistry. doi
  27. (1987). Biosensors: fundamentals and applications. doi
  28. (1997). Capillary isoelectric focusing of hemoglobin variants in the pediatric clinical laboratory. doi
  29. (2006). Capturing molecules with templated materials--Analysis and rational design of molecularly imprinted polymers. doi
  30. (2005). Carbon-nanotubes doped polypyrrole glucose biosensor. doi
  31. (2003). Characterization of hydrogen peroxide removal reaction by hemoglobin in the presence of reduced pyridine nucleotides. Biochimica Et Biophysica Acta - doi
  32. (2002). Chemical sensors and biosensors. doi
  33. (2005). Chemically modified chitosan beads as matrices for adsorptive separation of proteins by molecularly imprinted polymer. doi
  34. (2005). Comparison of fingerstick hemoglobin A1c levels assayed by DCA
  35. (2003). Construction of a molecular imprinting catalyst using target analogue template and its application for an amperometric fructosylamine sensor. doi
  36. (2005). Core-shell nanocluster films of hemoglobin and clay nanoparticle: Direct electrochemistry and electrocatalysis. doi
  37. (1999). Davidson's principles and practice of medicine.
  38. (2006). Development of a combined setup for simultaneous detection of total and glycated haemoglobin content in blood samples. doi
  39. (2000). Diabetes research. A guide for postgraduate. doi
  40. (1995). Diamond like carbon coated films for enzyme electrodes; characterization of biocompatibility and substrate diffusion limiting properties. doi
  41. (2005). Direct electrochemistry and electrocatalysis of heme proteins immobilised on gold nanoparticles stabilized by chitosan. doi
  42. (2001). Direct electrochemistry of hemoglobin in layer-by-layer films with poly(vinyl sulfonate) grown on pyrolytic graphite electrodes. doi
  43. (2002). Direct electron transfer between hemoglobin and a glassy carbon electrode facilitated by lipid-protected gold nanoparticles. doi
  44. (1999). Direct electron transfer for hemoglobin in biomembranelike dimyristoyl phosphatidylcholine films on pyrolytic graphite electrodes. doi
  45. (1998). Direct reduction of oxyhemoglobin on a bare glassy carbon electrode. doi
  46. (1996). Electrochemical activation of screen-printed carbon strips. doi
  47. (2007). Electrochemical detection of HbA1c, a maker for diabetes, using a flow immunoassay system. doi
  48. (2003). Electrochemical determination of human hemoglobin by using ferrocene carboxylic acid modified carbon powder microelectrode. doi
  49. (2010). Electrochemical dissolution of silver nanoparticles and its application in metalloimmunoassay. doi
  50. (2002). Electrochemical sensors based on molecularly imprinted polymers. doi
  51. (2002). Electron transfer of hemoglobin at electrodes modified with colloidal clay nanoparticles. doi
  52. (1999). Electropolymerized molecularly imprinted polymers as receptor layers in capacitive chemical sensors. doi
  53. (2005). Electrostatic adsorption of heme proteins alternated with polyamidoamine dendrimers for layer-by-layer assembly of electroactive films. doi
  54. (1995). Elements of biosensor construction. doi
  55. (2007). Encapsulation hemoglobin in ordered mesoporous silicas: Influence factors for immobilization and bioelectrochemistry. doi
  56. (2003). Enhanced peroxidase activity of hemoglobin in a DNA membrane and its application to an unmediated hydrogen peroxide biosensor. doi
  57. (2005). Fabrication of a biochip for percent hemoglobin A1c (%HbA1c) level measurement. doi
  58. (2006). Fabrication of a disposable biochip for measuring percent hemoglobin A1c (%HbA1c). Sensors and Actuators A: Physical 130-131, doi
  59. (2006). Fabrication of a Nanobiocomposite film Containing Heme Proteins and Carbon Nanotubes on a Choline Modified Glassy Carbon Electrode: Direct Electrochemistry and Electrochemical Catalysis. doi
  60. (2006). Ferroceneboronic acid-based amperometric biosensor for glycated hemoglobin. Sensors and Actuators B: doi
  61. (1997). Films of hemoglobin and didodecyldimethylammonium bromide with enhanced electron transfer rates. doi
  62. (1985). Free-radical production and oxidative reactions of haemoglobin. doi
  63. (2004). Global prevalence of diabetes. doi
  64. (1984). Glycosylated haemoglobin: Measurement and clinical use. doi
  65. (2005). Hemoglobin recognition by imprinting in semi-interpenetrating polymer network hydrogel based on polyacrylamide and chitosan. doi
  66. (2004). Highly sensitive voltammetric biosensor for nitric oxide based on its high affinity with hemoglobin. doi
  67. (1997). How to create polymers with protein-like capabilities: A theoretical suggestion. doi
  68. (2005). How to find effective functional monomers for effective molecularly imprinted polymers? Advanced Drug Delivery Reviews, doi
  69. (2006). Immobilization of hemoglobin on chitosan films as mimetic peroxidase. doi
  70. (2004). Immobilization of hemoglobin on zirconium dioxide nanoparticles for preparation of a novel hydrogen peroxide biosensor. doi
  71. (1999). Imprinted polymerbased sensor system for herbicides using differential- pulse voltammetry on screenprinted electrodes. doi
  72. (1999). In vitro diagnostics in diabetes: meeting the challenge.
  73. (2006). Influence of initiator and different polymerisation conditions on performance of molecularly imprinted polymers. doi
  74. (1993). Internal membranes and laminates for adaptation of amperometric enzyme electrodes to direct biofluid analysis. doi
  75. (2005). Investigation of protein imprinting in hydrogel-based molecularly imprinted polymers (HydroMIPs). Analytica Chimica Acta, doi
  76. (2003). Ivestigation of protein adsorption and electrochemical behaviour at a gold electrode. doi
  77. (2005). Langmuir monolayer approaches to protein recognition through molecular imprinting. doi
  78. (1991). Mediated amperometric enzyme electrode incorporating peroxidase for the determination of hydrogen peroxide in organic solvents. doi
  79. (2002). Membraneimmobilised haptoglobin as affinity matrix for a hemoglobin-A1c immunosensor. doi
  80. (2001). Metody pomiarow i kontroli jakosci w przemysle spozywczym. Akademia Rolnicza im Augusta Cieszkowskiego,
  81. (2003). Molecular imprinting catalyst based artificial enzyme sensor for fructosylamines. doi
  82. (2006). Molecular imprinting of polymers.
  83. (2006). Molecular imprinting science and technology: A survey of the literature for the years up to and including doi
  84. (1999). Molecularly Imprinted Electrosynthesized Polymers: New Materials for Biomimetic Sensors. doi
  85. (2006). Molecularly imprinted membranes for an improved recognition of biomolecules in aqueous medium. doi
  86. (2000). Molecularly imprinted polymers and their use in biomimetic sensors. doi
  87. (2007). Molecularly imprinted polymers for the recognition of proteins: The state of the art. doi
  88. (2006). Molecularly imprinted polymers in clinical diagnostics--Future potential and existing problems. doi
  89. (2004). Molecularly imprinted polypyrrolebased synthetic receptor for direct detection of bovine leukemia virus glycoproteins. doi
  90. (2003). Occupational methaemoglobinaemia: mechanisms of production, features, diagnosis and management including the use of methylene blue. doi
  91. (2009). On-chip type cation-exchange chromatography with ferrocene-labeled anti-hemoglobin antibody and electrochemical detector for determination of hemoglobin A1c level. doi
  92. (2007). Optimizing the formulation of a myoglobin molecularly imprinted thin-film polymer-formed using a micro-contact imprinting method. doi
  93. (2006). Performance of absorption photometry microchip for blood hemoglobin measurement integrated with processing circuits and doi
  94. (2005). Performance of an amperometric biosensor for the determination of hemoglobin. doi
  95. (1998). Performance of screenprinted carbon electrodes fabricated from different carbon inks. doi
  96. (1993). Performance of subcutaneously implanted needle-type glucose sensors employing a novel trilayer coating. doi
  97. (1994). Peroxidative activities of hemoglobin and hemoglobin derivatives. doi
  98. (1999). Poly(methylene blue) modified electrode sensor for haemoglobin. doi
  99. (2002). Polymer cookery: influence of polymerization conditions on the performance of molecularly imprinted polymers. doi
  100. (1995). Preparation and characterization of composite polymers exhibiting both selective molecular recognition and electrical conductivity.
  101. (1981). Preparation and use of a boronic acid affinity support for separation and quantitation of glycosylated hemoglobins. doi
  102. (1981). Preparation of derivatives of ferrous and ferric hemoglobin, doi
  103. (2006). Preparation of methylene bluedoped silica nanoparticle and its application to electroanalysis heme proteins. doi
  104. (2001). Probing the molecular basis for ligand-selective recognition in molecularly imprinted polymers selective for the local anaesthetic bupivacaine. doi
  105. (2004). Properties of poly-aminophenylboronate coatings in capillary electrophoresis for the selective separation of diastereoisomers and glycoproteins. doi
  106. (1995). Protein-boronic acid conjugates and their binding to low-molecular-mass cis-diols and glycated hemoglobin. doi
  107. (2005). Quartz crystal biosensor for detection of sugars and glycated hemoglobin. doi
  108. (2000). Reaction of hydrogen peroxide with ferrylhemoglobin: superoxide production and heme degradation. doi
  109. (2001). Research and development in biosensors. doi
  110. (2009). Review of haemoglobin A1c in the management of diabetes. doi
  111. (2003). Sensors/biosensors, based on screen-printing technology for biomedical applications.
  112. (1995). Separation of amino acids, peptides and proteins on molecularly imprinted stationary phases. doi
  113. (1996). Silica sol-gel immobilized amperometric biosensor for hydrogen peroxide. doi
  114. (2005). Standardization of HbA1c and optimal range of monitoring. doi
  115. (2005). Structured thin films as functional components within biosensors. doi
  116. (2001). Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays. doi
  117. (2007). Surface imprinted beads for the recognition of human serum albumin. doi
  118. (2001). Surface-grafted molecularly imprinted polymers for protein recognition. doi
  119. (1999). Templatemediated synthesis of a polymeric receptor specific to amino acid sequences. doi
  120. (1976). The biosynthesis of human hemoglobin A1C: slow glycosylation of hemoglobin in vivo. doi
  121. (1993). The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. The New England doi
  122. (1999). The haemoglobin enzyme.
  123. (1994). The influence of glycation on the peroxidase activity of haemoglobin. doi
  124. (2005). The interface behavior of hemoglobin at carbon nanotube and the detection for H2O2. doi
  125. (2006). The role of molecular recognition in regulating the catalytic activity of peroxidase-like polymers imprinted by a reductant substrate. doi
  126. (1998). The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin. doi
  127. (1997). The toxicities of native and modified hemoglobins. doi
  128. (2000). The use of immobilised templates - A new approach in molecular imprinting. Angewandte Chemie - doi
  129. (2005). Thirty years of haemoglobin electrochemistry. doi
  130. (2001). Towards molecularly imprinted polymers selective to peptides and proteins. The epitope approach. doi
  131. (2005). Towards the rational design of molecularly imprinted polymers. doi
  132. (2006). Using protein templates to direct the formation of thinfilm polymer surfaces. doi

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