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Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex

By Alfred E. Thumser, Aswir Abd Rashed, Paul A. Sharp and John K. Lodge

Abstract

It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration- dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+-ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+-ascorbate complex. (C) 2010 Elsevier Ltd. All rights reserved

Topics: Enterocyte iron uptake Ascorbate Ferric iron caco-2 cells nonheme iron colorimetric assay epithelial-cells expression transport absorption dmt1 bioavailability humans
Publisher: Elsevier Science B.V., Amsterdam.
Year: 2010
DOI identifier: 10.1016/j.foodchem.2010.04.031
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/4738
Provided by: Cranfield CERES
Journal:

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