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A label free electrochemical nanobiosensor study

By Juankun Zhang, Shanmu Dong, Jinhui Lu, Anthony P. F. Turner, Qingjie Fan, Shiru Jia, Hongjiang Yang, Changsheng Qiao, Hao Zhou and Guowei He

Abstract

Nano-porous silicon (PS) is an attractive material for incorporation into biosensors, because it has a large surface area combined with the ability to generate both optical and electrical signals. In this paper, we describe a label-free nanobiosensor for bovine serum albumin (BSA). Nano-porous silicon produced in our laboratory was functionalized prior to immobilization of anti-BSA antibody on the surface. Reaction with BSA in phosphate buffered saline (PBS) buffer resulted in an impedance change which was inversely proportional to the concentration of the analyte. The system PBS buffer/antigen-antibody/PS constitutes an electrolyte-insulator-semiconductor (EIS) structure, thus furnishing an impedance EIS nanobiosensor. The linear range of the sensor was 0-0.27 mg mL-1 and the sensitivity was less than 10 µg mL-1

Topics: Electrolyte-insulator-semiconductor, impedance, nano-porous silicon, nanobiosensor
Publisher: Taylor & Francis
Year: 2009
DOI identifier: 10.1080/00032710903201941
OAI identifier: oai:dspace.lib.cranfield.ac.uk:1826/4248
Provided by: Cranfield CERES
Journal:

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