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Postharvest investigations into chlorophyll fluorescence and low temperature injury in cut roses (Rosa hybrida L.)

By Nektarios E. Pompodakis


This is one of the first studies on the relationship between pre-harvest environmental conditions found in the Mediterranean and postharvest characteristics of cut roses (Rosa hybrida L. ). Effects of storage temperature on vase life parameters were also studied for roses grown all year round. The postharvest storage of roses at low temperature is a useful practice, in terms of market flow regulation. However, a reduction in vase life and loss of flower quality has been recorded after storage due to Low Temperature Injury (LTI). LTI of roses is difficult to assess by visual observation. Relative chlorophyll fluorescence (F'IFR, ), which is a non-invasive technique that provides an index of stress effects on photosystem II (PS II) activity, was used to investigate LTI in roses. The plant growth regulator abscisic acid (ABA) can cause physiological responses that protect plants against CI or LTI. The overall objectives of this study were firstly to evaluate the pre-harvest environmental conditions affecting vase life and secondly to evaluate novel potential ABA treatments to protect cut roses against LTI. Vase life durations and Fv. /Fm ratios measured after low temperature storage for `First Red' and `Akito' roses were seasonally dependant. Vase lives of roses grown during winter were significantly (P _< 0.001) shorter compared to roses grown during the rest of the year. In autumn and winter experiments F.. IF.. ratios were generally reduced following storage at 1°C, suggesting LTI of roses. Thus, the fall of F,. /Fm was due to an interaction of growing season and storage at I. T. However, in second year experiments, growing temperature and PFD were relatively higher and, as a result, Fý/Fm did not decline for `Akito' roses after low temperature storage, indicating a strong influence of environmental conditions. Higher PFD and temperature glasshouse during the year were positively and significantly correlated with maintenance of post-storage Fv. /Fm ratios and longer vase life. It is suggested that shorter vase lives and lower post-storage FV/Fm values after storage at 1°C are consequences of reduced photosynthesis and smaller carbohydrate pools in winter- harvested roses. Because of the lack of correlation between Fv. /Fm and post-storage vase life, it is concluded that the CF parameter F,. /Fm is not a practical index for assessing LTI in cold-stored roses. Growing roses in autumn and winter months increased flower blueing in red petals of `First Red' roses and prevented flower opening for both cultivars. ABA applied as pulse treatment before storage or as vase solution during vase life generally improved vase life parameters. Pulsing `Akito' roses with 10-1 M ABA before storage increased vase life and inhibited bent neck incidence. Also, the presence of ABA in vase solution increased vase life after storage at 1°C, reducing vase solution usage during vase life. Similarly, the synthetic ABA analogue PBI-365, as vase solution ingredient, was effective in extending vase life and reducing transpiration rates in roses after low temperature storage. Increased ABA levels were detected in leaves and petals using HPLC when roses were treated with exogenous ABA before storage and during vase life. Thus, it was assumed that ABA or PBI-365 acted on guard cells by causing stomatal closure. Electrolyte leakage and lipid peroxidation, measured after storage at 1°C, were markedly reduced by application of ABA. Both pulse ABA treatment and vase solutions containing ABA helped to recover F,, /Fm during vase life. Moreover, addition of PBI-365 in vase solution reduced the degree of lipid peroxidation in leaves and petals after storage at 1°C. These observations indicated a protection role of ABA against LTI for roses, which has also been observed in other crops. Further research at the cellular and/or molecular level may help in better understanding the physiological responses of roses to seasonal variation during the year and LTI. In addition, work is also required to look at the ABA and PBI-365 mode of action in roses. Additional research using a wide range of ABA concentrations and assays using exogenous radio-labelled ABA may help to better understand the nature of ABA efficacy

Publisher: Cranfield University
Year: 2005
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  2. (1995). 1-Methylcyclopropene inhibits ethylene action in cut phlox flowers.
  3. (2000). 1-Methylcyclopropene treatment efficacy in preventing ethylene perception in banana fruit and grevillea and waxflower flowers.
  4. (1997). 8'-acetylene ABA: An irreversible inhibitor of ABA 8'-hydroxylase.
  5. (1997). 8'-Methylene abscisic acid: An effective and persistent analog of abscisic acid.
  6. (1990). A chlorophyll fluorescence screening test to evaluate chilling tolerance in tomato.
  7. (1982). A possible role for abscisic acid in coupling stomatal conductance and photosynthetic carbon metabolism in leaves.
  8. (1989). A re-examination of stem blockage in cut roses.
  9. (1989). A review of research on postharvest physiology and horticulture of Australian native flowers.
  10. (1974). A slow-release chlorine compound as a vase water additive. Floricultural Review 154,63-65.
  11. (1996). A triazole compound extends the vase life of Geraldton waxflower.
  12. (1994). ABA and low temperature induce freezing tolerance via distinct regulatory pathways in wheat.
  13. (2002). ABA-based chemical signalling: the coordination of responses to stress in plants.
  14. (1988). Abscisic acid and water transport in sunflowers.
  15. (1994). Abscisic acid content of salt-stressed Phaseolus vulgaris L. - Comparison of highperformance-liquid-chromatography, gas-chromatography with electron-capture detection, enzyme-linked-immunosorbent-assay and radioimmunoassay.
  16. (1997). Abscisic acid-induced chilling tolerance in maize. In: Plant cold hardiness. Molecular Biology, biochemistry and physiology.
  17. (1991). Abscisic acid. In: Post-harvest physiology of perishable plant products,
  18. (1993). Abscission of flower peduncles in rose (Rosa x hybrida) plants. Scientia Horticulturae
  19. (1994). Acclimation, hydrogen peroxide, and abscisic acid protect mitochondria against irreversible chilling injury in maize seedlings.
  20. (1997). Advances in fertilization of Anthurium hybrid in
  21. (1984). Amelioration of chilling-induced water stress by abscisic acid-induced changes in root hydraulic conductance.
  22. (1973). An historical and critical review of postharvest physiology research on cut flowers.
  23. (1985). Analysis of percentage and rating scale data.
  24. (1993). Anthocyanins in modern roses: Chemical and colorimetric features in relation to the colour range.
  25. (2003). Antifungal compounds in Geraldton waxflower tissues.
  26. (2003). Antioxidant responses of tolerant and sensitive barley cultivars to boron toxicity.
  27. (2000). Assessing chilling tolerance in roses using chlorophyll fluorescence.
  28. (1991). Bacteria as a cause of vascular occlusion in cut fronds of Adiantrnn raddianuni: a scanning electron microscope study. Scientia Horticulturae 48,299-309.
  29. (1991). Biological bases of the use of cold in ornamental horticulture.
  30. (1981). Biosynthesis of stress ethylene induced by water deficit.
  31. (1984). Blue/UV light effects on anthocyanin synthesis. In: Blue light effects in biological systems
  32. (2003). Calcium distribution in cut roses as related to transpiration.
  33. (1981). Carbon dioxide concentration, photosynthesis, and dry matter production.
  34. (2001). Categories of petal senescence and abscission: A reevaluation.
  35. (2003). Changes in abscisic acid and phenols during flower development in two diverse species of rose.
  36. (1992). Changes in fluorescence lipid peroxidation products of room-ripened and vine-ripened tomato fruits in relation to other ripening parameters.
  37. (1998). Changes in stem diameter depend upon variations in water content in rose plants.
  38. (1978). Changes in the water status of cut roses and its relationships to bent-neck phenomenon.
  39. (1996). Characterisation of flower bud opening in roses; a comparison of `Madelon' and `Sonia' roses.
  40. (1993). Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves.
  41. (1978). Chemical pretreatments for China asters to increase flower longevity. Florists'
  42. (1992). Chilling damage of dark stored cucumbers (Cucumis sativus L. ) affects the maximum quantum yield of photosystem 2.
  43. (1994). Chilling injury induces lipis phase changes in membranes of tomato fruit.
  44. (1986). Chilling injury: A plea for uniform terminology.
  45. (1996). Chilling of maize seedlings: changes in water status and abscisic acid content in ten genotypes differing in chilling tolerance.
  46. (1995). Chilling-enhanced photooxidation: the production, action and study of reactive oxygen species produced during chilling in the light.
  47. (2000). Chlorophyll fluorescence -a practical guide.
  48. (1991). Chlorophyll fluorescence and photosynthesis: The basics.
  49. (1995). Chlorophyll fluorescence as a potential indicator of controlled-atmosphere disorders in `Marshall' McIntosh apples.
  50. (1984). Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals.
  51. (2003). Chlorophyll fluorescence: A general description and nomenclature,
  52. (1992). Chlorophyll fluorescence: a non-destructive method for detecting damage in the photosynthesis apparatus in plants.
  53. (1975). Chrysanthemum (Chrysanthenzuni morifoliunz Ramat. ): solution uptake and flower quality.
  54. (1994). Cold hardiness-specific glutathione reductase isozymes in red spruce.
  55. (1992). Cold storage of rose flowers (Rosa hybrida, cultivar `Visa'): physiological alternations. Scientia Horticulturae
  56. (1984). Cold storage of rose flowers: effects of cold storage and water loss on opening and vase life of `Mercedes' roses.
  57. (1991). Cold storage of selected members of the proteaceae and Australian native cut flowers.
  58. (2000). Cold-storageinduced changes in chlorophyll fluorescence of kangaroo paw Bush Dawn flowers.
  59. (1976). Combined effects of abscisic acid and sucrose on growth and senescence of rose flowers.
  60. (1977). Comparative photosynthesis of sun and shade plants.
  61. (1998). Competitive water relations between leaves and flower bud during transport of cut roses.
  62. (1976). Control of bacteria in vase water and quality of cut flowers as influenced by sodium dichloroisocyanurate, 1,3-dichloro-5,5-dimethylhydantoin and sucrose.
  63. (1995). Copper reverses silver inhibition of flower senescence in Petunia Nektarios E. Pompodakis Cranfield University, IBST PhD Thesis,
  64. (1979). Counteraction of bent-neck of cut roses with cobalt nitrate.
  65. (1967). Crop responses to water at different stages of growth.
  66. (1986). Cut flower longevity as affected by chemical pre-treatment.
  67. (2001). Cut rose flower longevity and its variation between plants of cv. Frisco grown in different lighting periods.
  68. (2000). Cut rose quality as affected by calcium supply and translocation.
  69. (1972). Decreasing water loss of cut roses with abscisic acid. HortScience 7,249.
  70. (1991). Definition of indicators of senescence in the rose: Effect of the application of plant hormones.
  71. (1995). Deleterious effects of sucrose in preservatives solutions on leaves of cut roses.
  72. (1994). Differential gene expression in chilling acclimated maize seedlings and evidence for the involvement of abscisic acid in chilling tolerance.
  73. (2001). Differentiation between petal blueing and senescence of cut `Mercedes' rose flowers.
  74. (2000). Discovering statistics using SPSS for
  75. (2000). Ectopic deposition of lignin in the pith of stems of two Arabidopsis mutants.
  76. (2001). Ectopic expression of ABIS gene enhances freezing tolerance in response to abscisic acid and low temperature in Arabidopsis tlzaliana.
  77. (1990). Effect of antimicrobial compounds on the number of bacteria in stems of cut rose flowers.
  78. (1991). Effect of bacterial suspensions on vascular occlusion in stems of cut rose flowers.
  79. (1981). Effect of Co 2+ and sucrose on the physiology of cut Samantha roses.
  80. (1996). Effect of cytokinins on axillary bud growth of `Madelon' roses.
  81. (1991). Effect of dry storage on bacterial counts in stems of cut rose flowers.
  82. (1994). Effect of dry storage on scape bending in cut Gerbera jamesonii flowers.
  83. (2001). Effect of irrigation and shading at the stage of flower bud appearance.
  84. (1997). Effect of light intensity on growth and quality of cut roses.
  85. (1979). Effect of millipore filtration, citric acid, and sucrose on peduncle water potential of cut rose flower.
  86. (2001). Effect of NaCl salinity and nitrogen fertilizer formulation on yield and nutrient status of roses.
  87. (1986). Effect of NH41N03 ratio in nutrient solution on growth and yield of greenhouse roses.
  88. (1995). Effect of postharvest factors on flower quality and longevity of cut carnations (Dianthus Caryophyllus L.
  89. (1989). Effect of pre-treatment and storage conditions on cut rose flowers.
  90. (1989). Effect of pretreatment and storage conditions on cut rose flowers.
  91. (1978). Effect of salinity in water supplies on greenhouse rose production.
  92. (1976). Effect of sucrose on response of cut carnation to kinetin, ethylene and abscisic acid.
  93. (1999). Effect of time since harvest and handling conditions on rehydration ability of cut chrysanthemum flowers. Postharvest Biology Nektarios E. Pompodakis Cranfield University, IBST PhD Thesis,
  94. (2001). Effects of air humidity and K: Ca ratio on growth, morphology, flowering and keeping quality of pot roses. Scientia Horticulturae 90,131-141.
  95. (2001). Effects of air humidity and K/Ca ratio in the nutrient supply on growth and postharvest characteristics of cut roses.
  96. (1998). Effects of air humidity, lighting period and lamp type on growth and vase life of roses.
  97. (1991). Effects of carbohydrate and water status on flower opening of cut `Madelon' roses.
  98. (1991). Effects of chilling on physiological responses and changes in hormone levels in two Euphorbia pulcherrima varieties with different chilling tolerance.
  99. (1989). Effects of ethylene on rose opening.
  100. (1986). Effects of moisture stress and senescence on the synthesis of abscisic acid in the primary leaves of bean.
  101. (1999). Effects of nitrogen nutrition on Sandersonia cut flower and tuber production in a soil-less medium.
  102. (1980). Effects of potassium and gibberellic acid on stem growth of whole sunflower plants.
  103. (1990). Effects of pre-harvest fertilization on the flower longevity of potted Campanula carpatica `Karl Foerster'. Scientia Horticulturae
  104. (2001). Effects of pre-harvest light intensity and temperature on carbohydrate levels and vase life of cut roses.
  105. (2005). Effects of seasonal variation and low temperature storage on vase life and leaf chlorophyll fluorescence of roses. Postharvest Biology and Technology,
  106. (1974). Effects of short-term heat and shade treatments on petal colour of `Baccara' roses.
  107. (2000). Effects of temperature and stem length on changes in carbohydrate content in summer-grown cut chrysanthemums during development and senescence.
  108. (2001). Effects of temperature on leaf area and flower size in rose.
  109. (1974). Effects of varying light intensities and temperature treatments applied to whole plants, or locally to leaves or flower buds, on growth pigmentation of `Baccara' roses.
  110. (2004). Effects of vase solution pH and abscisic acid on the longevity of cut `Baccara' roses.
  111. (1975). Effects of watering following a drought period on nitrogen metabolism of cut Iris germanica flowers.
  112. (1991). Effects of wetting agents and cut flower food on the vase life of cut roses.
  113. (1998). Effects of xylem pH on transpiration from wild-type and flacca tomato leaves. Plant Physiology 117,703-Nektarios E. Pompodakis Cranfield University, IBST PhD Thesis,
  114. (2001). Efficacy of inhibitors of ethylene binding in improvement of the postharvest characteristics of potted flowering plants.
  115. (1971). Electron microscopical studies of rose petal cells during flower maturation.
  116. (1997). Elevated CO2 mitigates chillinginduced water stress and photosynthetic reduction during chilling.
  117. (2003). Embolism in rose stems as a result of vascular occlusion by bacteria.
  118. (1989). Endogenous levels of abscisic acid in aging carnation flower parts.
  119. (1995). Enhanced ethylene synthesis in cold stored carnation flowers.
  120. (1989). Environmental influences on growth of ornamental plants.
  121. (1999). Environmental significance of anthocyanins in plant stress responses. Invited review.
  122. (1989). Ethylene and silver thiosulfate influence opening of cut rose flowers.
  123. (1999). Ethylene production of three lily species and their response to ethylene exposure.
  124. (2000). Evaluating yield and quality of roses with respect to nitrogen fertilization and leaf nitrogen status.
  125. (2001). Evaluation of long term transport of Colombian growth cut roses.
  126. (1978). Evaluation of postharvest handling methods for transcontinental truck shipments of cut carnations, chrysanthemums, and roses.
  127. (2000). Evidence for a wounding-induced xylem occlusion in stems of cut chrysanthemum flowers.
  128. (2000). Expression of genes for ethylene biosynthetic enzymes and an ethylene receptor in senescing flowers of miniature potted roses.
  129. (1981). Extension of vase life of cut marigold and chrysanthemum flowers by the use of cobalt chloride.
  130. (1987). Factors affecting the longevity of Aranda flowers. Acta Horticulturae 205,195-202,
  131. (1957). Flower preservatives: using silver and zinc ions as disinfectants. Florists'
  132. (1973). Fluorescent products of lipid peroxidation: I. Structural requirement for fluorescence in conjugated Schiff's bases.
  133. (1987). Freeze fracture evidence for lateral phase separations in the plasmalemma of chilling-injured avocado fruit.
  134. (1997). Growth and flower development in roses as affected by light.
  135. (1988). Growth and flowering in roses.
  136. (1995). High air humidity reduces the keeping quality of cut roses.
  137. (1990). Holding solutions for maximizing bud opening and vase-life of Dendrobium `Youppadeewan' flowers.
  138. (1977). Humidity fixed points of binary saturated aqueous solutions.
  139. (1990). Hydroxyquinoline citrate and low pH prevent vascular blockage in stems of cut rose flowers by reducing the number of bacteria.
  140. (1977). Hypobaric storage of perishable commodities-fruits, vegetables, flowers and seedlings.
  141. (1976). Identification and localization of vascular occlusions in cut roses.
  142. (1988). Identification of bacteria in the vase water of roses, and the effect of the isolated strains on water uptake. Scientia Horticidturae Nektarios E. Pompodakis Cranfield University, IBST PhD Thesis,
  143. (2002). Importance of abscisic acid for chilling tolerance of maize seedlings.
  144. (1988). Impregnating cut rose stems with nickel increases vase life. Current Research,
  145. (1964). Improved lasting life of `Velvet Times' roses with chemicals.
  146. (1998). Improving the longevity of cut roses by cool and wet transport.
  147. (2002). Influence of abscisic acid on flowering
  148. (1999). Influence of air humidity and lighting period on growth, vase life and water relations of 14 rose cultivars. Scientia Horticulturae 82,289-298.
  149. (1997). Influence of carbon dioxide enrichment on postharvest leaf chlorosis of miniature roses. Department of Horticulturae,
  150. (1998). Influence of high temperature on flower stem length and photosynthesis of roses.
  151. (1995). Influence of nitrogen supply on ABA levels and flower senescence in Rosa hybrida cv.
  152. (1971). Inhibition of vascular blockage and increased moisture retention in cut roses induced by pH, 8-Hydroxyquinoline citrate, and sucrose.
  153. (1975). Interrelationship of several phytohormones in the regulation of rose petal senescence.
  154. (1972). Interrelationships of ethylene and abscisic acid in the control of rose petal senescence.
  155. (1999). Involvement of ABA in postharvest life of miniature potted roses.
  156. (1989). Involvement of abscisic acid during water stress and recovery in petunia flowers.
  157. (2003). Involvement of abscisic acid in leaf and root of maize (Zea mays L. ) in avoiding chilling-induced water stress.
  158. (1992). Involvement of abscisic acid in regulating water status in Phaseolus vulgaris L. during chilling.
  159. (1991). Is vascular blockage in stems of cut lilac flowers mediated by ethylene? Acta Horticulturae
  160. (1993). Leaching losses of N from containergrown roses. Scientia Horticulturae 53,333-345.
  161. (1994). Lipid peroxidation and peroxide-scavenging Nektarios E. Pompodakis Cranfield University,
  162. (2000). Long term, low temperature storage injures kangaroo paw cut flowers.
  163. (1989). Low temperature-induced changes of abscisic acid contents in barley and cucumber leaves in relation to their water status.
  164. (1994). Maize chilling tolerance induction. In: Crop adaptation to cool climates.
  165. (1992). Maximizing the yield of greenhouse roses with respect to artificial lighting.
  166. (2001). Mechanisms of salt stress tolerance in two rose rootstocks: Rosa chinensis `Major'
  167. (1996). Membrane effects in postharvest physiology.
  168. (1991). Microorganisms at the cut surface and in xylem vessels of rose stems: a scanning Nektarios E. Pompodakis Cranfield University, IBST PhD Thesis,
  169. (1999). Mobility and effects on vase life of silver-containing compounds in cut rose flowers.
  170. (1988). Mode of action of cobalt extending the vase life of cut roses.
  171. (1999). Molecular analysis of acclimation to cold.
  172. (1989). Nonchemical methods for postharvest control of Botrytis cinerea on cut roses.
  173. (1992). Numbers of bacteria in aqueous solutions used for postharvest handling of cut flowers.
  174. (1974). Opposing effects of abscisic acid on senescence of rose flowers.
  175. (1993). Overexpression of superoxide dismutase protects plants from oxidative stress. Induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants.
  176. (1998). Oxidative events during programmed cell death of daylily (Hemerocallis hybrid) petals.
  177. (1996). Petal abscission in rose flowers: Effects of water potential, light intensity and light quality.
  178. (1999). PH as a stress signal.
  179. (1986). Phase transitions in liposomes formed from the polar lipids of mitochondria from chilling sensitive plants.
  180. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.
  181. (2001). Photosynthetic characteristics and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varieties differing in chilling sensitivity.
  182. (2000). Photosynthetic response of Japanese rose species Rosa bracteata and Rosa rugosa to temperature and light. Scientia Horticulturae 84,365-371.
  183. (1986). Physiological response of cut rose flowers to cold storage.
  184. (1984). Physiological responses of cut rose flowers to exposure to low temperature: Changes in membrane permeability and ethylene production.
  185. (1989). Physiology and biochemistry of flower petal senescence. Horticultural Review
  186. (1999). Plant Microtechnique and Microscopy.
  187. (1998). Plant Physiology (Book). Sinauer Associates,
  188. (2002). Polyamines and hyperhydricity in micropropagated carnation plants.
  189. (2004). Pompodakis Cranfield University,
  190. (2004). Pompodakis Cranfield University, IBST PhD Thesis,
  191. (1996). Postharvest characteristics of cut Camellia japonica L. `Kumasaka'. Postharvest Biology
  192. (1988). Postharvest characteristics of Geraldton waxflowers.
  193. (1993). Postharvest characteristics of native Australian flowers.
  194. (1993). Postharvest floral organs fall in Geraldton tvaxflower (Chamelauci«ni uncinatum Schauer).
  195. (1990). Postharvest handling and storage of cut flowers, florist greens, and potted plants.
  196. (2002). Postharvest handling of nine specialty cut flower species. Scientia Horticulturae 92,293-303.
  197. (1993). Postharvest quality and water relations of Bouvardia cut flowers as affected by preharvest temperature and season.
  198. (2001). Postharvest responses of different ornamental products to preharvest nitrogen supply: Role of carbohydrates, photosynthesis and plant hormones.
  199. (1986). Postharvest studies of cut Zantedeschia inflorescences.
  200. (1991). Postharvest studies on Syringa vulgaris.
  201. (1995). Pre- and postharvest treatments on vase life of `Sonia' and `Madelon' roses.
  202. (2001). Preservative solutions containing boric acid delay senescence of carnation flowers.
  203. (1989). Production results of the carnation cultivated in a cold greenhouse.
  204. (1977). Prolongation of vase life of Lupinus harttivegii Lindl. by chemical treatments.
  205. (2001). Rapid low temperature-induced stomatal closure occurs in cold-tolerant Comnielina con nnrnis leaves but not in coldsensitive tobacco leaves, via a mechanism that involves apoplastic calcium but not abscisic acid.
  206. (1991). Regulation of ABA levels is senescing petals of rose flowers.
  207. (2000). Regulation of cell death in flower petals.
  208. (1998). Relation between nitrogen and soluble carbohydrate concentrations and subsequent rooting of chrysanthemum cuttings as influenced by nitrogen nutrition of stock plants and cool-storage of cuttings.
  209. (1989). Relation of chilling stress to membrane permeability.
  210. (1996). Relationship between cavitation and water uptake in rose stems.
  211. (1992). Reporting of objective colour measurements.
  212. (2000). Responses of native Australian cut flowers to treatment with 1-Methylcyclopropene and ethylene.
  213. (1983). Rising atmospheric CO2 and crop productivity.
  214. (1997). Role of abscisic acid in chilling tolerance of rice (Oryza sativa L. ). II Modulation of free polyamine levels.
  215. (1988). Role of carbohydrates in diurnal chilling sensitivity of tomato seedlings.
  216. (1989). Role of endogenous bacteria in vascular blockage of cut rose flowers.
  217. (1991). Role of ethylene in vascular blockage in cut fronds from the fern Adiannrm raddianum. Scientia Horticulturae 46,161-169.
  218. (1961). Rose neck droop.
  219. (1990). Screening for stress tolerance by chlorophyll fluorescence,
  220. (2001). Seasonal changes in vase life, transpiration and leaf drying of cut roses.
  221. (2000). Selection of biocides for use in floral preservatives.
  222. (2001). Selection of rose cultivars for low light greenhouse production by photosynthetic features.
  223. (1990). Semi-automated method for the determination of abscisic acid in crude plant extracts.
  224. (1979). Senescence and postharvest physiology of cut flowers, Part 1. Horticultural Reviews
  225. (1981). Senescence and postharvest physiology of cut flowers, Part 2. Horticultural Reviews
  226. (1986). Senescence in cut carnation flowers: Temporal and physiological relationships among water status, ethylene, abscisic acid and membrane permeability.
  227. (1995). Senescence of Broccoli buds is related to changes in lipid peroxidation.
  228. (1973). Senescence of cut carnation flower: respiration and sugar status.
  229. (1993). Sensitivity of stomata to abscisic Nektarios E. Pompodakis Cranfield University,
  230. (1979). Some characteristics of water flow through isolated rose stem segments.
  231. (2000). Steady-state and maximum chlorophyll fluorescence responses to water stress in grapevine leaves: A new remote sensing system. Remote Sensing of Environment
  232. (1987). Stomatal behaviour and leaf water potential of chilled and waterstressed Solanum melongena, as influenced by growth history.
  233. (1983). Stomatal behaviour and water relations of chilled Phaseolus vulgaris L. and Pisuni sativu m L.
  234. (2002). Stomatal control by chemical signalling and the exploitation of this mechanism to increase water use efficiency in agriculture.
  235. (1986). Storage environment related stresses and flower senescence.
  236. (1990). Superoxide dismutase, catalase, atocopherol content of stored potato tubers.
  237. (1996). Synthesis metabolism and biological activity of a Nektarios E. Pompodakis Cranfield University,
  238. (1974). The action of kinetin in improving the water balance and delaying senescence processes of cut rose flowers.
  239. (1970). The cause of bent neck in cut roses.
  240. (1998). The effect of drought hardening and chilling on ABA content in xylem sap and ABA delivery rate from root of tomato plant.
  241. (1995). The effect of field application of K on postharvest behaviour of gladiolus.
  242. (1993). The effect of germicides on the longevity of cut flowers.
  243. (1975). The effect of growing temperature on keeping quality of cut roses.
  244. (2001). The effect of phosphorus nutrition on growth, flowering and leaf nutrient concentrations of osteospermum.
  245. (1969). The effect of temperature and light on growth and flowering of Rosa `Baccara' in greenhouses.
  246. (2001). The effect of transient water stress on sugar metabolism and development of cut flowers.
  247. (1986). The effect of water stress on ethylene production and ethylene sensitivity of freesia inflorescences.
  248. (1987). The effects of leakage of substances from mechanically wounded rose stems on bacterial growth and flower quality.
  249. (1990). The effects of microbial exopolysaccharides (EPS) in vase water on the water relations and the vase life of Rosa cv.
  250. (1977). The effects of some chemical treatments on leaf water conductance of cut flowering stems of Chrysanthemum morifolitan.
  251. (1989). The effects on the vase life of cut Rosa cultivar `Sonia' of bacteria added to the vase water. Scientia Horticulturae 39,167-179.
  252. (1988). The infiltration ability of micro-organisms Bacillus, Fusarium, Kluyveronryces and Pseudomonas spp. into xylem vessels of Gerbera. cv. `Fleur' and Rosa cv. `Sonia' cut flowers: a scanning electron microscope study.
  253. (1995). The influence of light quality on rose flower senescence: involvement of abscisic acid.
  254. (1995). The influence of nitrogen on keeping quality of pot plants.
  255. (1989). The influence of purified microbial pectic enzymes on the xylem anatomy, water uptake and vase life of Rosa cultivar `Sonia'. Scientia Horticulturae
  256. (1982). The involvement of water stress and ethylene in senescence of cut carnation flowers.
  257. (1976). The mechanism of chill- and drought-hardening of Pheseolus vulgaris leaves.
  258. (1992). The mode of action of bacteria in the vascular occlusion of cut rose flowers.
  259. (1991). The relationship between petal water potential and levels of abscisic acid in rose flower.
  260. (1975). The role of abscisic acid in senescence of detached tobacco leaves.
  261. (2003). The role of abscisic acid in the response of a specific vacuolar invertase to water stress in the adult maize leaf.
  262. (1999). The role of calcium on several aspects of plant and flower quality from a floricultural perspective.
  263. (1975). The role of flower and leaves in cut flower water uptake. Floricultural Review 156,24-25.
  264. (1974). The role of flower and leaves in cut flower water uptake. Scientia Horticulturae
  265. (1990). The use of chlorophyll fluorescence in assessment of low temperature hardiness in blackcurrant (Ribes nigrum L.
  266. (1983). The water relations of Maidenhair fronds treated with silver nitrate. Scientia Horticulturae
  267. (1996). Toxicity to peptides to bacteria present in the vase water of cut roses.
  268. (1985). Transient water stress in carnation flowers: Effect of amino-oxyacetic acid.
  269. (1976). Treatments to improve water balance of cut flowers.
  270. (1989). Treatments to prevent flower abscission in Geraldton waxflower.
  271. (1994). Ultrasonic acoustic emissions from excised Nektarios E. Pompodakis Cranfield University, IBST PhD Thesis,
  272. (1997). Uptake, translocation and fractionation of nitrogen in narcissus organs by using 15N.
  273. (1969). Vascular blockage, water absorption stomatal opening and Nektarios E. Pompodakis Cranfield University,
  274. (1994). Vascular occlusion in cut flowering rose stems exposed to air: Role of water entry into the lumina of the xylem conduits opened by cutting.
  275. (1995). Vascular occlusion in cut rose flowers: A survey.
  276. (1995). Vascular occlusion in stems of cut rose flowers exposed to air: Role of xylem anatomy and rates of transpiration.
  277. (1980). Water balance of cut and intact `Sonia' rose plants.
  278. (1992). Water balance of the foliage of cut Geraldton waxflower.
  279. (2001). Water loss and post-harvest characteristics of cut roses grown at high or moderate relative air humidity. Scientia Horticulturae 89,217-226.
  280. (1978). Water relations and keeping-quality of cut gerbera flowers. I. The cause of stem break.
  281. (1995). Water relations and senescence of cut Iris flowers: effects of cycloheximide.
  282. (1992). Water relations of cut chrysanthemum flowers.
  283. (1993). Water relations of cut flowering branches of Thryptomene calycina (Lindl.
  284. (1997). Water relations of cut flowers.
  285. (1992). Water relations of cut rose flowers cv. Sonia after dry storage.
  286. (1990). Whole plant net photosynthesis of miniature roses influenced by light, C02, and temperature.
  287. (1997). Xylem sap pH increase: a drought signal received at the apoplastic face of the guard cell which involves the suppression of saturable ABA uptake by the epidermal symplast.
  288. (1993). Xylem-transported abscisic acid: the relative importance of its mass and its concentration in the control of stomatal aperture.
  289. (1997). Year-round supplementary lighting at two photosynthetic photon flux densities for cut roses.