Article thumbnail
Location of Repository

Investigating the in situ degradation of atrazine in groundwater.

By Robert Pearson, Andrew R. Godley and Elise Cartmell


This study focused on whether or not atrazine could be degraded by indigenous groundwater bacteria as part of an in situ remediation approach. Groundwater was taken from an unconfined middle upper chalk site where concentrations of atrazine and nitrate were typically in the ranges 0.02-0.2 µg litre-1 and 11.6-25.1 mg NO3-N litre-1 respectively. Sacrificial batch studies were performed using this groundwater spiked with atrazine at a concentration of 10 µg litre-1 in conjunction with a minimal mineral salts liquid (Glu-MMSL) medium which contained glucose as the sole carbon source. Treatments comprised either the Glu-MMSL groundwater cultured bacteria or Pseudomonas sp. strain ADP. Results from sacrificial batches indicated the occurrence of bacterial growth and denitrification as monitored by optical density (absorbance at 600 nm) and NO3-N content. Analysis of atrazine content by solid phase extraction coupled with high-performance liquid chromatography showed no degradation of atrazine over a period of 103 days in either treatment. These results indicated that no acclimatised bacterial community featuring positive degraders to the herbicide atrazine had become established within this chalk aquifer in response to the trace levels encountered. Copyright © 2006 Society of Chemical Industry

Publisher: Wiley
Year: 2006
DOI identifier: 10.1002/ps.1161
OAI identifier:
Provided by: Cranfield CERES

Suggested articles


  1. (2002). Aamand A and Bjerg PL, Effect of exposure history on microbial herbicide degradation in an aerobic aquifer affected by a point source. doi
  2. (2001). Atrazine degradation under denitrifying conditions in continuous culture of Pseudomonas sp. strain ADP. doi
  3. (2001). Atrazine: environmental contamination and ecological effects.
  4. (2000). Bioaugmentation of atrazine and fenamiphos impacted groundwater: laboratory evaluation. doi
  5. (1995). Biodegradation of the herbicide atrazine in groundwater under laboratory conditions. doi
  6. (1983). Biological significance and fate of atrazine under aquifer conditions.
  7. (2000). Biotransformation of pesticides in saturated-zone materials. JH y d r o l8:89–103 doi
  8. Changes in the enantiomeric fraction as evidence of natural attenuation of mecoprop in a limestone aquifer. JC o n t a mH y d r o l doi
  9. (2000). Characterisation of atrazine degradation and nitrate reduction by Pseudomonas sp. strain ADP. doi
  10. Degradation of herbicides in two sandy aquifers under different redox conditions. doi
  11. (2002). Degradation of the (R)- and (S)-enantiomers of the herbicides MCPP and dichloroprop in a continuous field-injection experiment. doi
  12. (2003). Enantioselective biodegradation of mecoprop in aerobic and anaerobic microcosms. doi
  13. (2001). Impacts of atrazine in aquatic ecosystems. doi
  14. Isolation and characterisation of a Pseudomonas sp that mineralises the s-triazine herbicide atrazine.
  15. (2001). Isolation from agricultural soil and characterisation of a Sphingomonas sp able to mineralise the phenylurea herbicide isoproturon. doi
  16. (2000). isoproturon and atrazine in and above a sandy aquifer: vertical distribution ofmineralisationpotential.EnvironSciTechnol 34:2426–2430 doi
  17. (1996). Occurrence and determination of pesticides in natural and treated waters. doi
  18. (2002). oling WFM and vanVerseveld HW, Natural attenuation: what does the subsurface have in store?
  19. (2000). Potential for isoproturon, atrazine and mecoprop to be degraded within a chalk aquifer system. JC o n t a mH y d r o l44:1–18 doi
  20. Sorption and degradation of the herbicide 2-methyl-4,6-dinitrophenol under aerobic conditions in a sandy aquifer in Vejen, doi
  21. (1996). Strategies for chromatographic analysis of pesticide residues in water. doi
  22. (2003). The natural (baseline) quality of groundwater: a UK pilot study. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.