The egt gene of the baculovirus Autographa californica nuclear polyhedrosis virus encodes an ecdysteroid UDP-glucosyltransferase. The glucoside formed by this enzyme using ecdysone and UDP-glucose as substrates has been purified and structurally characterized by nuclear magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry. These studies have identified the conjugate as ecdysone 22-O-beta-D-glucopyranoside. Substrate specificity studies have confirmed that ecdysteroids lacking a hydroxyl moiety at C-22 are not substrates for the enzyme
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