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High performance liquid chromatography (HPLC) in the investigation of gout in paleopathology.

By D.J. Swinson, J. Snaith, Jo Buckberry and M.B. Brickley

Abstract

noGout is a disease caused by the abnormal accumulation of uric acid in the body, which can\ud result in sodium urate crystals forming tophi at joints, with associated erosion of bone and\ud cartilage. Only two examples of tophi have been reported from archaeological individuals, and\ud the diagnosis of gout based on dry bone manifestations can be difficult. This paper presents\ud preliminary results of a new technique to aid the diagnosis of gout in palaeopathology, namely\ud high performance liquid chromatography (HPLC). Five archaeological skeletons with suspected\ud gout (diagnosed using visual and radiological analysis) and three controls were\ud analysed. Two of the gouty individuals had a white powder in their erosive lesions. HPLC\ud showed the presence of uric acid in bone in four of the five individuals with evidence of gouty\ud arthritis and was negative for uric acid in bone from the three controls. The white powder was\ud also positive for uric acid. With reliance on the presence of articular erosions, cases of gout will\ud be missed in archaeological human bone. HPLC measurement of uric acid could prove useful\ud in the differential diagnosis of erosive arthropathy in archaeology. It may also be useful in\ud identifying individuals with an increased body pool of uric acid, linked to conditions included in\ud the term `metabolic syndrome┬┐. As a result, HPLC uric acid measurement also has the potential\ud to provide additional information on health and lifestyle in past communities

Topics: Liquid chromatography, Palaeopathology, Gout, Uric acid, HPLC, Metabolic syndrome, Tophi, Skeletal remains
Year: 2010
DOI identifier: 10.1002/oa.1009
OAI identifier: oai:bradscholars.brad.ac.uk:10454/4683
Provided by: Bradford Scholars
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