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Membrane Type MMPs Show Differential Expression in Non-Small Cell Lung Cancer (NSCLC) Compared to Normal Lung; Correlation of MMP-14 mRNA Expression and Proteolytic Activity.

By Jennifer M. Atkinson, Jason H. Gill, Paul M. Loadman, Sandie W. Martin, J. Pennington, V.A. Anikin, A.J. Mearns and D.R. Edwards

Abstract

NoImproved understanding of the involvement of matrix metalloproteinases (MMPs), including membrane-type MMPs (MT-MMPs), in human tumours has potential diagnostic, prognostic and therapeutic implications. We assessed the relationship between MT-MMP expression and clinicopathological parameters in human non-small cell lung cancer (NSCLC) and histologically normal lung tissue by quantitative Real Time PCR (qRT-PCR). All MT-MMPs (MMPs 14-17, 24 and 25) were detected by qRT-PCR with significantly higher MMP-14, -15 and -17 expression observed in tumour relative to normal lung specimens. MMP-16 was undetectable in normal lung but expressed in 8% tumours. MMP-15 demonstrated significant overexpression in adenocarcinomas relative to squamous cell carcinomas and normal lung tissue. MMP-14 mRNA expression strongly correlated to MMP-14 proteolytic activity in preclinical tumour models, indicating that qRT-PCR may predict MMP-14 activity levels in NSCLC. These data suggest that MMP-14, -15 and -17 may be good markers of disease, or therapeutic targets for treatment of human NSCLC

Topics: Membrane-type metalloproteinases, Lung cancer, Real-time PCR, Carcinoma, Non-small cell lung, Cancer therapeutics
Year: 2007
DOI identifier: 10.1016/j.ejca.2007.05.009
OAI identifier: oai:bradscholars.brad.ac.uk:10454/3494
Provided by: Bradford Scholars
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